基本概况
姓名:金双侠[1]
硕博导:博导
职称:教授
开设课程:植物生物技术，作物生产实习，育种学实习
研究方向:棉花生物技术，细胞质基因组及生物技术[2]
个人简介
华中农业大学植物科学技术学院作物遗传育种专业博士，教授，博士生导师，作物遗传改良国家重点实验室PI。2001-2006年，在华中农业大学植物科学技术学院攻读硕士、博士学位，获农学博士学位. 2007.10-2012.4在美国宾夕法尼亚大学Henry Daniell教授指导下进行博士后研究，研究方向为植物叶绿体转化。当前主要从事棉花生物技术研发（基因编辑等）；棉花与害虫互作分子机制及抗虫相关基因的克隆和高效表达载体（叶绿体转化表达体系）的开发研究。[2]
近年来以第一作者或通讯作者在植物学知名期刊Trends in Plant Science、New phytologist、Plant Physiology、Plant Biotechnology Journal等杂志上发表20多篇植物抗虫基因工程的学术文章，其中IF > 6的文章10篇，总引用次数1300多次。2018年入选Plant Biotechnology Journal杂志（IF=6.3）编委以及Journal of Cotton Research（棉花学报英文版）副主编 (高级编辑）；2017年当选国际棉花基因组协会（ICGI）共同主席。先后主持国家自然科学基金面上项目3项，转基因专项子课题1项，十三五国家重点研发专项（子课题）1项，湖北省自然科学基金重点项目（杰青）1项，华中农业大学人才培植项目2项。[2-3]
科研项目
十三五国家重点研发专项 子课题 主持 2016-2020[3]
转基因专项子课题 主持 2016-2020[3]
湖北省自然科学基金重点项目（杰青项目） 主持,2015.1-2016.12[2]
国家自然科学基金 烟粉虱与棉花互作的分子机理研究 主持,2014-2017
国家自然科学基金 植物凝集素基因在棉花中的功能分析,主持,2012-2015
新疆建设兵团博士资金项目 转基因抗病虫、抗逆聚合杂交棉新组合选育技术研究,主持,2007-2009
国家自然科学基金 棉花外源基因叶绿体转化体系的建立及其应用(30771368),主持,2008-2010
教育部博士点新教师基金 以gfp基因为报告基因监测转基因棉花花粉漂移(2007.0504087),主持,2008-2010
国家自然科学基金重大国际合作项目 Development of strong pest-resistance cotton via transforming
plastid and its application in breeding,主要参加者,2009-2011；
国家自然科学基金 利用基因诱捕构建棉花功能基因组研究突变群体,主要参加,2005-2007[2]
发明专利及获奖情况获奖情况
获奖情况
【棉花种质创新及强优势杂交新品种选育与应用】 国家科技进步二等奖 2013年[4]
【棉花体细胞离体遗传操作技术与控制理论】 教育部自然科学二等奖
湖北省优秀博士学位论文（第10批）获奖者,2008年
湖北省优秀本科论文指导奖，2008年。指导的2007界本科生张碧瑶，其论文：新疆棉花高体细胞胚胎发生
能力基因型的筛选，获得湖北省优秀本科学位论文
“棉花分子育种技术体系建立与应用”获2008年湖北省技术发明二等奖
获第十、十一届湖北省自然科学优秀论文一等奖2次[3]
申报六项发明专利
超声波辅助农杆菌转化棉花胚芽的方法，专利申请号：200710063650.0
一种分离棉花叶绿体DNA的方法，专利申请号：200710052665.7
利用苦豆子分离基因SaVP1培育耐高温拟南芥的方法. 专利号 ZL 201310006089.8
利用花花柴KcNHX1 基因培育耐高温拟南芥的方法 专利申请号： 201310160491.1
利用棉花GhPEPC基因创造棉花高油材料的方法 专利申请号：201510836230.6
一种提高棉花再生及转化效率的方法及应用 专利申请号：201510836230.6
一种棉花基因的编辑方法 专利申请号 2016111884024[3]
一种DsRED2报告基因在棉花遗传改良中的应用 专利申请号 201611113581.5[3]
发表的代表性论文：
[1]Li,Jianying;Manghwar,Hakim;Sun,Lin;Wang,Pengcheng;Wang,Guanying;Sheng,Hanyan;Zhang,Jie;Liu,Hao;Qin,Lei;Rui,Hangping;Li,Bo;Lindsey,Keith;Daniell,Henry;Jin,Shuangxia*;Zhang,[3]Xianlong.Wholegenomesequencingrevealsrareoff-targetmutationsandconsiderableinherentgeneticor/andsomaclonalvariationsinCRISPR-Cas9-editedcottonplants.Plant Biotechnology Journal, accepted.（*通讯作者） (SCI IF:6.3)[2]
[2] Jianying Li, Maojun Wang, Yajun Li, Qinghua Zhang, Keith Lindsey, Henry Daniell,Shuangxia Jin*, Xianlong Zhang. Multi-omics analyses reveal epigenomics basis for cotton somatic embryogenesis through successive regeneration acclimation (SRA) process, 2018,Plant Biotechnology Journal, DOI:10.1111/pbi.12988（*通讯作者） (SCI IF:6.3)[1]
[3] [3]Luo, Jing; Liang, Sijia; Li, Jianying; Xu, Zhongping; Li, Lun; Zhu, Bangqin; Li, Zhe; Lei, Chaoliang; Lindsey, Keith; Chen, Lizhen*; Jin, Shuangxia*; Zhang, Xianlong. A transgenic strategy for controlling plant bugs (Adelphocoris suturalis) through expression of double- stranded RNA (dsRNA) homologous to Fatty acyl-CoA reductase (FAR) in cotton. New Phytologist,DOI:10.1111/nph.14636, 2017（*通讯作者） (SCI IF: 7.2)[1]
[4] Lin Sun, Muna Alariqi, YiZhu, Jianying Li, Zelin Li, Qing Wang, Yajun Li, Hangping Rui, Xianlong Zhang,Shuangxia Jin*. Red fluorescent protein (DsRed2), an ideal reporter for cotton genetic transformation and molecular b reeding,Crop Journal, 2018, DOI:10.1016/j.cj.2018.05.002 （*通讯作者） (SCI IF:2.6)
[5] Wang, Pengcheng; Zhang, Jun; Sun, Lin; Ma, Yizan; Xu, Jiao; Liang, Sijia; Deng, Jinwu; Tan, Jiafu; Zhang, Qing; Tu, Lili; Daniell, Henry; Jin, Shuangxia*; Zhang, Xianlong*. High efficient multi-sites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system,Plant Biotechnology Journal, DOI: 10.1111/pbi.12755, 2017,（*通讯作者） (SCI IF: 6.3)
[6] Maojun Wang, Lili Tu, Min Lin, Zhongxu Lin, Pengcheng Wang, Qingyong Yang, Zhengxiu Ye, Chao Shen, Jianying Li, Lin Zhang, Xiaolin Zhou, Xinhui Nie, Zhonghua Li, Kai Guo, Yizan Ma, Cong Huang, Shuangxia Jin, Longfu Zhu, Xiyan Yang, Ling Min, Daojun Yuan, Qinghua Zhang, Keith Lindsey & Xianlong Zhang,2017, Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication，Nature Genetics, 49(4):579-587[1]
[6] Maojun Wang, Lili Tu, Min Lin, Zhongxu Lin, Pengcheng Wang, Qingyong Yang, Zhengxiu Ye, Chao Shen, Jianying Li, Lin Zhang, Xiaolin Zhou, Xinhui Nie, Zhonghua Li, Kai Guo, Yizan Ma, Cong Huang, Shuangxia Jin, Longfu Zhu, Xiyan Yang, Ling Min, Daojun Yuan, Qinghua Zhang, Keith Lindsey & Xianlong Zhang,2017, Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication，Nature Genetics, 49(4):579-587[1]
[7] Jianying Li#, Lizhen Zhu#, Joe Hull, Sijia Liang, Henry Daniell, Shuangxia Jin*, Xianlong Zhang. Transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly). Plant Biotechnology Journal, 2016, DOI: 10.1111/pbi.12554. (SCI, IF:6.3）(通讯作者）
[8] Zhongping Xu , Jingwen Li , Xiaoping Guo , Shuangxia Jin*, Xianlong Zhang*.Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference.Scientific Reports, 2016, DOI: 10.1038/srep33342.（*通讯作者）(SCI IF: 5.2)[1]
[8] Zhongping Xu , Jingwen Li , Xiaoping Guo , Shuangxia Jin*, Xianlong Zhang*.Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference.Scientific Reports, 2016, DOI: 10.1038/srep33342.（*通讯作者）(SCI IF: 5.2)[1]
[9] Shuangxia Jin and Henry Daniell.The Engineered Chloroplast Genome Just Got Smarter. Trends in Plant Science, 2015，20(10):622-640 （SCI,IF:12.93)
[10] Geng Tian, Linlin Cheng, Xuewei Qi, Zonghe Ge, Changying Niu, Xianlong Zhang, Shuangxia Jin* Transgenic Cotton Plants Expressing Double-stranded RNAs Target HMG-CoA Reductase (HMGR) Gene Inhibits the Growth, Development and Survival of Cotton Bollworms. Int. J. Biol. Sci. 2015; 11(11):1296-1305.(SCI, IF:4.5)（通讯作者）[1]
[10] Geng Tian, Linlin Cheng, Xuewei Qi, Zonghe Ge, Changying Niu, Xianlong Zhang, Shuangxia Jin* Transgenic Cotton Plants Expressing Double-stranded RNAs Target HMG-CoA Reductase (HMGR) Gene Inhibits the Growth, Development and Survival of Cotton Bollworms. Int. J. Biol. Sci. 2015; 11(11):1296-1305.(SCI, IF:4.5)（通讯作者）[1]
[11] Jin Shuanxia,Singh, Dolendro,Li Lebin,Zhang, Xianlong and Daniell, Henry. Engineered chloroplast dsRNA silences cytochrome p450 monooxygenase, V-ATPase and chitin synthases genes in the insect gut and disrupts Helicoverpa armigera larval development and pupation. Plant Biotechnology Journal, 2015,13 (3), 435-446.(SCI, IF:6.3)
[12] Shuangxia Jin and Henry Daniell.Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stress by reducing reactive oxygen species. Plant Biotechnology Journal, 2014 (12),1274-1285 . (SCI, IF:6.3)[1]
[12] Shuangxia Jin and Henry Daniell.Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stress by reducing reactive oxygen species. Plant Biotechnology Journal, 2014 (12),1274-1285 . (SCI, IF:6.3)[1]
[13] Shuangxia Jin, Xianlong Zhang1, Henry Daniell. Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, lepidopteran insects, bacterial and viral pathogens. Plant Biotechnology Journal 2012,10(3):313-327 (Cover Story, IF 6.3).
[14] Shuangxia Jin, Anderson Kanagaraj, Dheeraj Verma, Theo Lange, Henry Daniell.Release of Hormones from Conjugates: Chloroplast Expression of ß-glucosidase Results in Elevated Phytohormone Levels Associated with Significant Increase in Biomass and Protection from Aphids or Whiteflies Conferred by Sucrose Esters. Plant Physiology ,2011,155,222-235.(SCI，IF:6.5)[1]
[14] Shuangxia Jin, Anderson Kanagaraj, Dheeraj Verma, Theo Lange, Henry Daniell.Release of Hormones from Conjugates: Chloroplast Expression of ß-glucosidase Results in Elevated Phytohormone Levels Associated with Significant Increase in Biomass and Protection from Aphids or Whiteflies Conferred by Sucrose Esters. Plant Physiology ,2011,155,222-235.(SCI，IF:6.5)[1]
- 参考资料
- 1.金双侠 - 师资队伍 - 棉花遗传改良团队．棉花遗传改良团队[引用日期2018-09-20]
- 2.华中农业大学师资队伍．华中农业大学植物科技学院师资队伍[引用日期2015-03-19]
- 3.师资信息．华中农业大学植物科学技术学院[引用日期2016-09-03]
- 4.2013国家科学进步奖获奖名单．中华人民共和国科学技术部[引用日期2016-01-17]
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