想请教NC,LNA都代表什么意思????? mir-34a表达的检测,,那么对应的mir-nc,mir-lna 和mir-34a等关系都是什么,个代表什么呀,,荧光素酶,强度越高就是结合的越多吗?
求请教,双荧光素酶实验结果图怎么解读呢想请教NC,LNA都代表什么意思????? mir-34a表达的检测,,那么对应的mir-nc,mir-lna 和mir-34a等关系都是什么,个代表什么呀,,荧光素酶,强度越高就是结合的越多吗? 打赏 |
LNA: locked nucleic acid. Try to use the link: https://en.wikipedia.org/wiki/Locked_nucleic_acid for more details. LNA is nothing to do luciferase assay. miR-LNA indicates micRNA is synthesized with LNA modification. In the figure, C is a result to normalize different synthesized microRNA or negative control (NC) exprerssion in testing cell lines, such as BT549, etc. D, a kind of result of luciferase assay upon different micRNA or NC transfection. |