我们用TriZol从细胞里面提出了DNA,然后配了0.5%的琼脂糖胶,用TAE配的,电压120V,30min,跑出来的结果如图:想知道哪一条是跑出来的DNA,这样的结果能用来做DNA甲基化芯片吗?谢谢大家!
哪一个条带可能是全基因组DNA?我们用TriZol从细胞里面提出了DNA,然后配了0.5%的琼脂糖胶,用TAE配的,电压120V,30min,跑出来的结果如图:想知道哪一条是跑出来的DNA,这样的结果能用来做DNA甲基化芯片吗?谢谢大家!
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In sample lane the most fast-running bands could be RNA, the very slow running bands at the top (larger than 16 kb) could be genomic DNA, the signals staggered in the well, junk. The smear, could be from junk or from degraded genomic DNA contaminated in sample preparation. |
mpark Thanks very much ! |