赛默飞世尔 Dharmacon^® RNAi 和 Pierce^® 蛋白检测专题讲座

题目1: Staying On Target with siRNAs: Issues and Solutions

主讲：Elizabeth L. Godden, Ph.D.

(Dharmacon.Field Application Specialist)

语言：英文

题目2: Western Blotting的问题与对策

主讲：王世春博士

(赛默飞世尔科技·生命科学部 技术支持经理)

语言：中文

时间和地点：

广州地区：2008-3-17 星期一 上午9:30-11:30

         中山大学北校区永生楼第七教室

上海地区：2008-3-18 星期二 上午9:30-11:30

         上海市肇嘉浜路500号（岳阳路口）

         上海好望角大饭店周仁厅（5F）

北京地区：2008-3-19 星期三 上午9:30-11:30

          中国医学科学院基础医学研究所老科研楼8层东阶梯教室

主办：赛默飞世尔科技·生命科学部  （010-80499033）

广州莱德尔生物科技有限公司  （020-32057085）

上海皓嘉科技发展有限公司    （021-61028980）

北京北方同正生物技术发展公司（010-63441426）

讲座结束后有精美礼品赠送

 

Staying On Target with siRNAs: Issues and Solutions

Abstract: siRNA-mediated RNA interference (RNAi) has become a key tool in functional genomics. In this presentation, we will describe how a combination of (1) the first siRNA design algorithm, (2) pooling strategy, and (3) unique chemical modifications ensures high potency of chemically synthesized siRNAs.  

We will demonstrate how sequence alignment algorithms (e.g.BLAST and Smith-Waterman) have limited ability to accurately filter siRNAs with potential off-target effects. Moreover, we will show that a primary source of off-target effects is derived from the “seed” region (nucleotides 2-8) of the siRNA’s antisense (guide) strand recognizing the 3’-UTR of an unintended target, resulting in silencing via a microRNA-like mechanism.  Finally, we will present strategies that significantly reduce sequence-associated off-targets thus minimizing the incidence of false phenotypes.

The experimental approaches derived from these studies are expected to dramatically improve the quality and reliability of RNAi applications.

Topics presented in this seminar:

•  Introduction to RNAi biology

•  Strategies to ensure the function and specificity of siRNA sequences

•  Transfections

•  Use of appropriate controls