求助慢病毒载体问题...

为什么？他告诉你原因了吗

genecreate

为什么？他告诉你原因了吗

The chemical you are studying associates with cis element in CMV promoter, it may affect promoter transcription activity, but has nothing to do with mutations of transgene. This is why the company told you that the compoany was not able to make it.

If you want to know whether the chemical you are studying can modify the transgene product function, you need to know first if the chemical stimulates or suppresses that function or not, then you should elucidate how the chemical affects transgene product function by examining function activity of mutant forms of transgene product.

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mpark

The chemical you are studying associates with cis element in CMV promoter, it may affect promoter transcription activity, but has nothing to do with mutations of transgene. This is why the company told you that the compoany was not able to make it.

If you want to know whether the chemical you are studying can modify the transgene product function, you need to know first if the chemical stimulates or suppresses that function or not, then you should elucidate how the chemical affects transgene product function by examining function activity of mutant forms of transgene product.

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做实验本来就是试验，可以构建到同一个载体试试

可以和编码区一起构建，只是这个公司可能没有相应的病毒载体可用而已。

ps：微信公众号knockgene，擅长解决慢病毒相关各种技术难题，若有疑问，欢迎上面交流