Try to make 1:3 dilution of the Marker wqith loading buffer, and add 3 ul of the diluted Marker along with your samples and run the gel at 60 V and less than 70 mA in 1 X TAE for 60 minutes.
Try to make 1:3 dilution of the Marker wqith loading buffer, and add 3 ul of the diluted Marker along with your samples and run the gel at 60 V and less than 70 mA in 1 X TAE for 60 minutes.