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举报
2014-05-08 00:18
缓冲液提供合适的pH值，提供足够的带电离子。也许SDS还能弥补转膜过程中从蛋白肽链上脱落的部分SDS。甲醇可增加PVDF膜对蛋白的吸附能力。
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举报
2014-05-08 19:12
Methanol is only necessary if using nitrocellulose. If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. |
已解决
