展开引用
phychosh
NME1 F BamHI: TTAGGATCCaccATGaagcgacctgccgccacaaagaaggctggacaggctaagaagaagaaa ATGGCCAACTGTGAG
NME1 R XhoI: GCACTCGAG TTAAGCATAATCTGGAACATCATATGGATA TTCATAGATCCAGTTCT
其中上游引物带有核定位信号NLS序列,下游引物带有HA tag序列。请问NLS序列翻译的是哪些氨基酸及蛋白质???
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很奇怪,你自己都标出了NLS序列啊
aagcgacctgccgccacaaagaaggctggacaggctaagaagaagaaa 48bp
所翻译的aa就是
Lys Arg Pro Ala Ala Thr Lys Ala Gly Gln Ala Lys Lys Lys Lys