全基因组测序找到相关乙醇脱氢酶基因...

Please provide your reason why you want to do whole genome sequencing to find alcohol dehydrogenase in budding yeast?

mpark

Please provide your reason why you want to do whole genome sequencing to find alcohol dehydrogenase in budding yeast?

It is not neccssary doing whole genome sequencing to fish out the enzyme gene. Try to retrieve the gene DNA sequences in NCBI gene bank and design primers to run PCR using yeast genomic DNA as template.

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mpark

It is not neccssary doing whole genome sequencing to fish out the enzyme gene. Try to retrieve the gene DNA sequences in NCBI gene bank and design primers to run PCR using yeast genomic DNA as template.

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Try to run test PCR or Southern hybridization first using known DNA sequences derived from other yeast strains.

mpark

Try to run test PCR or Southern hybridization first using known DNA sequences derived from other yeast strains.

It is dependent on the research funding. The whole genome sequencing would cost you over $100,000.00, while classical method less than $10,000.00. The gene is not a novel one, and there are many ways to clone it.

可以通过基因注释的结果来找到的

mao081312

可以通过基因注释的结果来找到的