点击图片查看原图

SignaGen转染试剂 for L929 Cell

产品价格: ¥1680.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国山东济南市
有效期至:
长期有效
最后更新:
2021-05-16 01:30:02
浏览次数:
26
企业信息

产品详情

品牌名称:
SignaGen
货号: SL100489-L929
保存条件: 4°C
供应商: 济南美中清水湾生物科技有限公司
数量: 现货 大量
英文名: GenJet™ In Vitro DNA Tranfection Reagent for L929 Cell
保质期: 一年
规格: 毫升/支
Description
GenJet™ DNA In Vitro Tranfection Reagent for L929 cell is pre-optimized for transfecting L929 cells. L929 is a murine aneuploid fibrosarcoma cell line which is often used to assay TNF-alpha and TNF-beta. Treatment with TNF initiates apoptosis and subsequent cell death.  The level of detection is on the order of 50 pg/mL. Another cell line which is approximately 5-10-fold more sensitive is WEHI-164. Some L929 subclones have been developed that allow detection of TNF at concentrations of approximately 30 pmol/L (approximately 500 fg/mL). Sublines that are resistant to TNF have been established also. 

Refer to the following optimal transfection conditions for maximal transfection efficiency on L929 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)

~80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter) 
65%
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet 
 
温馨提示:不可用于临床治疗。

免责声明

本网页所展示的有关【SignaGen转染试剂 for L929 Cell】的信息/图片/参数等由的会员【济南思科生物科技有限公司 】提供,由【生物实验采购网】会员【济南思科生物科技有限公司 】自行对信息/图片/参数等的真实性、准确性和合法性负责,本平台(本网站)仅提供展示服务,请谨慎交易,因交易而产生的法 律关系及法律纠纷由您自行协商解决,本平台(本网站)对此不承担任何责任。您在本网页可以浏览【SignaGen转染试剂 for L929 Cell】有关的信息/图片/价格等及提供 【SignaGen转染试剂 for L929 Cell】的商家公司简介、联系方式等信息。

在您的合法权益受到侵害时,请您致电,我们将竭诚为您服务,感谢您对【生物实验采购网】的关注与支持!

网站首页 | 关于我们 | 联系方式 | 使用协议 | 版权隐私 | 网站地图  |  排名推广  |  广告服务  |  积分换礼  |  网站留言  |  RSS订阅  |  违规举报
 
免责声明:本站有部分内容来自互联网,如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权,请来电或致函告之,本网站将在规定时间内给予删除等相关处理。