货号: | SL100568-MEF |
供应商: | 美中清水湾生物科技有限公司 |
数量: | 现货 大量 |
英文名: | GenMute™ siRNA Transfection Reagent for MEFs |
保质期: | 1年 |
保存条件: | 4°C |
规格: | 毫升 |
Description:
GenMute™ siRNA Transfection Reagent for MEFs is pre-optimized for transfecting siRNA to Mouse Embryonic Fibroblasts (MEFs). MEFs are required to support the growth of undifferentiated mouse or human ES cells, iPS cells, thus they are also called feeder cells. MEFs are isolated from mouse embryos and are used at their early passages. Before MEFs are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing.
Refer to the following optimal transfection conditions for maximal silencing on MEFs. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
Summary of Optimal Transfection Conditions: |
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Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.