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GenMute™ siRNA Transfection Reagent for MCF7 Cell

产品价格: ¥1980.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国山东济南市
有效期至:
长期有效
最后更新:
2021-05-29 01:30:05
浏览次数:
77
企业信息

产品详情

品牌名称:
SignaGen
货号: SL100568-MCF7
供应商: 美中清水湾生物科技有限公司
数量: 现货 大量
英文名: GenMute™ siRNA Transfection Reagent for MCF7 Cell
保质期: 1年
保存条件: 4°C
规格: 毫升

Description:
GenMute™ siRNA Transfection Reagent for MCF7 Cell is pre-optimized for transfecting siRNA to MCF7 cellsMCF-7 cell is a breast cancer cell line was isolated in 1970 from a 69-year-old Caucasian woman. This cell line retained several characteristics of differentiated mammary epithelium including the ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. This cell line expresses the oncogene Wnt7B. Tumor necrosis factor alpha (TNF alpha) inhibits the growth of MCF-7 breast cancer cells. Treatment with anti-estrogens can modulate the secretion of insulin-like growth factor binding protein''s.

Size & content:
- GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
- GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution

Refer to the following optimal transfection conditions for maximal silencing on MCF7 cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute
 Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency 


Transfection Results:
Reporter Gene
Silencing Efficiency (% )


DMEM with 4.5 g/L glucose, 10% FBS
~50%
40 nM

1xGenMute™ Transfection Buffer
~15 minutes at RT
48 hours


Renilla Luciferase
90%


Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol.pdf
温馨提示:不可用于临床治疗。

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