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GenMute™ siRNA Transfection Reagent for Insulinoma

产品价格: ¥1980.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国山东济南市
有效期至:
长期有效
最后更新:
2021-05-27 01:30:02
浏览次数:
96
企业信息

产品详情

品牌名称:
SignaGen
货号: SL100568-INS
供应商: 美中清水湾生物科技有限公司
数量: 现货 大量
英文名: GenMute™ siRNA Transfection Reagent for Insulinoma
保质期: 1年
保存条件: 4°C
规格: 毫升

Description:
GenMute™ siRNA
 Transfection Reagent for Insulinoma (INS) cell is pre-optimized for transfecting siRNA to INS. Insulinoma cell is a tumor cell line derived from pancreatic beta cells.  Normal beta cells secrete insulin in response to increases in blood glucose. The resulting increase in insulin acts to lower blood glucose back to normal levels at which point further secretion of insulin is stopped. In contrast, the secretion of insulin by INS cell is not properly regulated by glucose and the tumors will continue to secrete insulin causing glucose levels to fall further than normal. 

Size & content:
- GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 25 pmoles siRNA or miRNA mimics in 24-well plate
- GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution

Refer to the following optimal transfection conditions for maximal silencing on INS cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute
 Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency 


Transfection Results:
Reporter Gene
Silencing Efficiency (% )


RPMI 1640 with 4.5 g/L glucose, 10% FBS
~60%
50 nM

1xGenMute™ Transfection Buffer
~15 minutes at RT
48 hours


Renilla Luciferase
80%


Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol.pdf
温馨提示:不可用于临床治疗。

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