货号: | SL100568-HepG2 |
供应商: | 美中清水湾生物科技有限公司 |
数量: | 现货 大量 |
英文名: | GenMute™ siRNA Transfection Reagent for HepG2 Cell |
保质期: | 1年 |
保存条件: | 4°C |
规格: | 毫升 |
Description:
GenMute™ siRNA Transfection Reagent for HepG2 Cell is pre-optimized for transfecting siRNA to HepG2 cells. HepG2 (Hepatocellular carcinoma, human) is a perpetual add cell line which was derived from the liver tissue of a 15 year old caucasian male with a well differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a model chromosome number of 55 and are not tumorigenic in nude mice. The cells secrete a variety of major plasma proteins e.g.. albumin, alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. HepG2 cells have been shown to be G418 resistant (400µg/mL). The cells will respond to stimulation with human growth hormone.
Refer to the following optimal transfection conditions for maximal silencing on HepG2 cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
Summary of Optimal Transfection Conditions: |
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Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.