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PREMO FUCCI CELL CYCLE
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伯乐BIO-RADGOAT ANTI| 货号： | E1910 |
| 供应商： | 北京利华开顺贸易有限公司 |
| 保存条件： | -20℃ |
| 规格： | 100 assays |
其它组分：
描述：
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| Dual-Luciferase® Reporter (DLR™) Assay System (Dual-Luciferase® 双萤光素酶报告基因检测系统) 为双报告基因检测提供有效的手段。 在DLR™ 检测中，萤火虫(Photinus pyralis ) 萤光素酶和海肾(Renilla reniformis ) 萤光素酶的活性可在单个样品中依次检测。首先检测萤火虫萤光素酶，将萤光素酶检测试剂II(Luciferase Assay Reagent II, LAR II)加入样品中，产生的光信号至少持续1分钟。定量萤火虫萤光强度后，再在同一个样品中加入Stop & Glo® 试剂，将上述反应终止，同时启动海肾萤光素酶反应。使用带有试剂自动注射器的发光检测仪，可在4 秒内完成两个检测。在DLR™ 检测系统中，两个报告基因产生的线性检测的灵敏度均可达埃摩尔级(<10-18)，在实验宿主细胞内均无内源活性。而且，DLR™ 检测的一体化模式既可快速定量检测转染细胞，也可用于快速定量检测无细胞转录/ 翻译反应体系中的两个报告基因。 |
原厂资料：
The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at least one minute. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding Stop & Glo® Reagent to the same sample. Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors. In the DLR™ Assay System, both reporters yield linear assays with attomole (<10–18) sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ Assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.
For best results with the Dual-Luciferase® Assay, Promega recommends using a luminometer that has been validated for use with the assay. These luminometers are qualified as DLReady™. For a listing of qualified instruments, please visit the DLReady™ Validated Luminometers page.
The pGL4 Luciferase Reporter Vectors are designed for use with the DLR™ Assay Systems. A Renilla luciferase vector with constitutive expression may be used in combination with any experimental firefly luciferase vector to co-transfect mammalian cells.
Notice for Cat.# E1960 and E1980: Sufficient Passive Lysis Buffer is provided to perform 1,000 assays with cells grown in 96-well plates (typically 20μl of 1X PLB per well). For applications requiring more lysis reagent (e.g., >100μl/well), additional Passive Lysis Buffer may be purchased separately.
Features - Benefits
- Greater Accuracy: Renilla luciferase internal control allows more accurate results.
- Convenience: Samples don't have to be split; saves plates and time.
- Sensitivity: Allows study of weak promoters, low-level expression/regulation and expression in cells that transfect poorly.
- Linearity: Range extends 7 logs; very active samples typically do not need dilution.
Applications
For more information, see the Protocols & Applications Guide.
注意事项：
| 储存条件 | 储存于-20℃。 |
详细说明
| 冷链运输标志: | 储存于-20℃。 |
| 准确性更高：: | 使用海肾萤光素酶做内对照可得到更精确的结果。 |
| 方便：: | 无需分开样品，可节省平板和时间。 |
| 灵敏：: | 可用于研究弱启动子，低水平表达/ 调控和转染不佳的细胞的表达。 |
| 线性范围：: | 线性范围超过7 个数量级；活性很高的样品一般无需稀释。 |
