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Oxoid 现货|Liver Broth|肝肉汤|现货

产品价格: ¥1947.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国上海
有效期至:
长期有效
最后更新:
2021-04-10 01:30:01
浏览次数:
37

产品详情

品牌名称:
Oxoid
货号: CM0077B
供应商: 上海善然生物科技有限公司
数量: 现货
英文名: Liver Broth
保存条件: 低温
相关信息:  

产品名称:肝肉汤 Liver Broth
供应商:Oxoid
货号:CM0077
规格:500g
产品说明:
Directions

Suspend 64 grams in 1 litre of distilled water and soak for 15 minutes, with occasional stirring. Distribute into 18mm diameter tubes to a depth of 50mm so that the bottom of the tube is filled with liver particles. Agitate frequently during distribution to keep the liver tissue in suspension
Technique
Gillespie in communication with Scarr1 recommended Liver Broth for the examination of canners’ sugar for hydrogen swells caused by thermophilic anaerobes (Clostridium thermosaccharolyticum). A 20% w/v solution of the sugar is steamed for 30 minutes to destroy vegetative forms and inoculated into Liver Broth sealed with agar. The standard proposed was a maximum of 1 positive tube in six - with 20ml inocula incubated for 72 hours at 56°C.
This medium should be made up only when required for use. Storage of the reconstituted medium is not recommended because air may be absorbed and the re-steaming necessary for the restoration of anaerobic conditions darkens the medium. Liver broth is not an homogeneous medium; consisting of a layer of liver particles and a cloudy supernatant. Growth produces an obvious increase in turbidity and some organisms (e.g. Clostridium thermosaccharolyticum) also produce gas which often pushes the agar plug towards the top of the tube. Some organisms also digest the solid liver tissue.
Appearance 
Dehydrated medium: Brown powder / granules 
Prepared medium: Dark brown solution / particles
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8° C.

保存条件: RT
温馨提示:不可用于临床治疗。

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