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磷酸酶抑制剂Cocktail

产品价格: ¥222.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国上海
有效期至:
长期有效
最后更新:
2020-11-07 02:00:02
浏览次数:
94

产品详情

品牌名称:
Biotool
货号: B15001
供应商: 上海善然生物科技有限公司
数量: 现货
保存条件: 低温
规格: 1 mL A + 1mL B

For Phosphorylation Protection, more inhibitors mean better results. So we provide Two Tubes with 8 inhibitors.
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. For decades, researchers have used easily-accessible phosphate mimetics (tube A) to prevent the loss of phosphorylation on biomolecules in vitro. In recent years however, a number of naturally derived agents (tube B) have been discovered as potent inhibitors of phosphatases, thus increasing the available protection spectrum.

Contents

Tube # Ingredient 100x Conc. Target
A (Aqueous) Sodium Fluoride 100 mM Acid phosphatases
Sodium Orthovanadate 100 mM Alkaline phosphatases, PTPs, ATPases
Sodium Tartrate 400 mM Acid phosphatases
Sodium Molybdate 115 mM Acid and phosphoprotein phosphatases
Imidazole 200 mM Alkaline phosphatases
B (DMSO) (-)-p-Bromotetramisole oxalate 2.5 mM Alkaline phosphatases
Cantharidin 500 μM Ser/Thr phosphatases
Microcystin LR, Microcystis aeruginosa 500 nM PP1 and PP2A

Works well in common detergents such as 1% SDS, Triton, and NP-40
DTT, EDTA and EGTA may reduce the protection effects of Sodium Orthovanadate

储存条件

The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.

实验方法

Application Fields Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Experiment Protocol Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Note:
For optimum protection of phosphorylated proteins and lipids, we recommend simultaneous use of BOTH Tubes A and B. Because the inhibitor cocktails target different families of phosphatases, synergistic stabilization effects are observed with dual-treatment.

The Phosphatase Inhibitor Cocktail does not contain protease inhibitors. Therefore,we recommend that a protease inhibitor cocktail is added beforehand. Protease inhibitor cocktails are frequently used in mammalian cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.

温馨提示:不可用于临床治疗。

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