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焦碳酸二乙酯(液体)E1| 货号： | B15001 |
| 供应商： | 上海善然生物科技有限公司 |
| 数量： | 现货 |
| 保存条件： | 低温 |
| 规格： | 1 mL A + 1mL B |
For Phosphorylation Protection, more inhibitors mean better results. So we provide Two Tubes with 8 inhibitors.
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. For decades, researchers have used easily-accessible phosphate mimetics (tube A) to prevent the loss of phosphorylation on biomolecules in vitro. In recent years however, a number of naturally derived agents (tube B) have been discovered as potent inhibitors of phosphatases, thus increasing the available protection spectrum.
Contents
| Tube # | Ingredient | 100x Conc. | Target |
|---|---|---|---|
| A (Aqueous) | Sodium Fluoride | 100 mM | Acid phosphatases |
| Sodium Orthovanadate | 100 mM | Alkaline phosphatases, PTPs, ATPases | |
| Sodium Tartrate | 400 mM | Acid phosphatases | |
| Sodium Molybdate | 115 mM | Acid and phosphoprotein phosphatases | |
| Imidazole | 200 mM | Alkaline phosphatases | |
| B (DMSO) | (-)-p-Bromotetramisole oxalate | 2.5 mM | Alkaline phosphatases |
| Cantharidin | 500 μM | Ser/Thr phosphatases | |
| Microcystin LR, Microcystis aeruginosa | 500 nM | PP1 and PP2A |
Works well in common detergents such as 1% SDS, Triton, and NP-40
DTT, EDTA and EGTA may reduce the protection effects of Sodium Orthovanadate
储存条件
The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.
实验方法
| Application Fields | Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc. |
| Experiment Protocol | Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition. |
Note:
For optimum protection of phosphorylated proteins and lipids, we recommend simultaneous use of BOTH Tubes A and B. Because the inhibitor cocktails target different families of phosphatases, synergistic stabilization effects are observed with dual-treatment.
The Phosphatase Inhibitor Cocktail does not contain protease inhibitors. Therefore,we recommend that a protease inhibitor cocktail is added beforehand. Protease inhibitor cocktails are frequently used in mammalian cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.
