货号: | C11885500BT |
CAS号: | 详询 |
供应商: | 弗尔博生物 |
数量: | 500ML |
保存条件: | 2-8°避光 |
规格: | 500ML |
产品型号:C11885500BT
产品用途:用于科学研究及细胞体外培养,不用于临床诊断。
DMEM, high glucose, pyruvate
Description
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With Without
• High Glucose • HEPES
• L-glutamine
• Phenol Red
• Sodium Pyruvate
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Product use
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco® DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
Specifications
Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
Glucose: High Glucose
Serum Supplementation: Standard Serum Supplementation
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Product Size: 10 x 500 mL
Volume (Metric): 500 ml
Shipping Condition: Room Temperature
温馨提示:不可用于临床治疗。