Human Ubiquitin-conjugating enzyme E2 D2 (UBE2D2) ELISA Kit

货号：	AE63019HU
样本：	Serum, Plasma, Other biological fluids
标记物：	P62837
适应物种：	Human (Homo sapiens)
应用：	Quantitative
检测方法：	Sandwich
检测限：	Request Information
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：UBE2D2
别名：E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH5B; ubiquitin carrier protein|ubiquitin-conjugating enzyme E2 D2|ubiquitin-conjugating enzyme E2-17 kDa 2|ubiquitin-conjugating enzyme E2D 2|ubiquitin-conjugating
检测范围：Request Information
产品概述：The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.Ubiquitin-conjugating enzyme E2 D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. UBE2D2 has been shown to interact with PJA1, Baculoviral IAP repeat-containing protein 3, PJA2, UBE3A and NEDD4.
特异性：This assay has high sensitivity and excellent specificity for detection of Human UBE2D2. No significant cross-reactivity or interference between Human UBE2D2 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Human UBE2D2 and the recovery rates were calculated by comparing the measured value to the expected amount of Human UBE2D2 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human UBE2D2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate UBE2D2 in samples. An antibody specific for UBE2D2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2D2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2D2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2D2 bound in the initial step. The color development is stopped and the intensity of the color is measured.