Rat Protein max (MAX) ELISA Kit

货号：	AE34167RA
样本：	Serum, Plasma, Other biological fluids
标记物：	P52164
适应物种：	Rat (Rattus norvegicus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	Request Information
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：MAX
别名：MGC10775; MGC11225; MGC18164; MGC34679; MGC36767; bHLHd4; bHLHd5; bHLHd6; bHLHd7; bHLHd8; orf1; MAX protein|helix-loop-helix zipper protein|myc-associated factor X
检测范围：Request Information
产品概述：MAX is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Unlike MYC, the steady state level of MAX RNA was not significantly modulated with respect to proliferation or differentiation. Unlike MYC RNA, MAX RNA was relatively stable with a half-life of more than 3 hours, and therefore it did not exhibit the characteristic short half-life of RNAs encoded by most immediate early genes.
特异性：This assay has high sensitivity and excellent specificity for detection of Rat MAX. No significant cross-reactivity or interference between Rat MAX and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Rat MAX and the recovery rates were calculated by comparing the measured value to the expected amount of Rat MAX in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat MAX and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate MAX in samples. An antibody specific for MAX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAX bound in the initial step. The color development is stopped and the intensity of the color is measured.