货号: | EM0821 |
保存条件: | -20℃ |
供应商: | 华夏远洋生物商城 |
数量: | 大量现货或国外期货3-4周 |
英文名: | CpG Methyltransferase (M.SssI) |
保质期: | 一年 |
规格: | 250 units |
Hazardous No
Formulation One µL of M.SssI protects 1 µg of genomic DNA from digestion with HpaII in 15 min at 37°C in 50 µL of recommended reaction buffer.
Quality Control Prolonged incubation
No detectable change in DNA fragment band pattern observed following agarose gel electrophoresis of SmaI linearized pUC19 DNA incubated for 16 hours with 1 µL of M.SssI.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of single-stranded or double-stranded labeled oligonucleotides observed after incubation with 1 µL of M.SssI for 4 hours at 37°C, analysis by polyacrylamide gel electrophoresis and phosphoimaging.
Storage Buffer CpG Methyltransferase (M.SssI) is supplied in 10 mM potassium phosphate (pH 7.0 at 25°C), 400 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, and 50% glycerol.