货号: | K0441 |
供应商: | 北京生东科技有限公司 |
规格: | 50T |
Highlights
• Exceptionally high yields—up to 200 µg in 2 hours
• Versatile—suitable for both short and long RNA transcripts
• Milligram amounts of RNA in a single, scaled-up reaction
• Flexible—generates unlabeled, labeled or capped RNA
• RiboRuler RNA Ladder supplied with kit for sizing and quantification
Applications
• In vitro transcription
• In vitro translation
• Generation of hybridization probes for:
• microarrays
• in situ hybridization
• blotting
• RNase protection assays
• RNA binding protein assays
• Antisense RNA and RNAi
• RNA amplification
• Microinjection studies
Includes
• TranscriptAid Enzyme Mix
• 5X TranscriptAid Reaction Buffer
• DNase I, RNase-free
• ATP Solution
• CTP Solution
• GTP Solution
• UTP Solution
• Control template
• 3 M Sodium Acetate Solution, pH 5.2
• DEPC-treated Water
• 2X RNA Loading Dye
• RiboRuler High Range RNA Ladder, ready-to-use
• 0.5 M EDTA, pH 8.0
• Detailed Protocol
* The improved version of TranscriptAid T7 High Yield Transcription Kit contains Tris-buffered NTPs instead of previously used NTPs titrated with KOH. Usage of Tris-buffered NTPs leads to higher yields of RNA transcripts.