胶原酶Ⅱ

货号：	C8150
CAS号：	9001-12-1
供应商：	索莱宝
数量：	大量
英文名：	CollagenaseⅡ
保质期：	2年
保存条件：	-20℃
规格：	100mg

胶原酶Ⅱ
别名：梭菌肽酶 A
英文名称：CollagenaseⅡ
CAS：9001-12-1
储存条件：-20℃
纯度：0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
外观（性状）：棕色或棕褐色结晶性粉末
单位：支
 胶原酶Ⅱ适用于肝脏，骨，甲状腺，心脏和唾液腺组织细胞的分离。

胶原蛋白酶 来源于溶组织梭菌

Collagenase from Clostridium histolyticum

release of physiologically active rat epididymal adipocytes tested, Type II, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Caution

As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.

Preparation Note

Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Related Categories    3.4.x.x Peptidases, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Cell Dissociation,

Cell Dissociation and Cell Lysis, Cell Signaling Enzymes, Collagenases, Core Bioreagents, Crude Collagenase, Use-Tested, Cytoskeleton and Extracellular Matrix, Enzyme Class Index, Enzymes, Enzymes for Diagnostic Kit Manufacturing, Extracellular Matrix, Extracellular Matrix Peptides and Proteins, Histology, MMP Enzymes, Matrix Metalloproteinases Products, Other Apoptosis Related Reagents, Proteases, Proteases & Protein Sequencing, Proteolytic Enzymes, Proteolytic Enzymes and Substrates, Research Essentials, 生化试剂, 细胞信号转导和神经科学, 细胞生物学, 酶、抑制剂和底物

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suitability       release of physiologically active rat epididymal adipocytes tested

Featured Industry       Diagnostic Assay Manufacturing

shipped in       wet ice

storage temp.       −20°C