货号: | HZ0008 |
供应商: | 上海沪震生物科技有限公司 |
数量: | 大量 |
保质期: | 1年 |
保存条件: | -20摄氏度保存 |
pcDNA3.1(hyg)+ / pCDNA3.1-Hygro(+) / pcDNA3.1/Hygro(+)产品信息
订购信息
货号 | 载体名称 | 出品公司 | 载体用途 | 价格 | 到货周期 |
HZ0008 | pcDNA3.1(hyg)+ / pCDNA3.1-Hygro(+) / pcDNA3.1/Hygro(+) | 哺乳动物表达载体 | 1000 900 | 现货 |
产品参数
载体用途:哺乳动物表达载体
原核抗性:Ampicillin(氨苄青霉素)
真核筛选标记:Hygromycin(潮霉素)
启动子:CMV promter
表达水平:高
5' 测序引物:CMV-f
3' 测序引物:BGH-r
载体拷贝数:高
载体描述
This pcDNA3.1/Hygro(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Hygromycin selectable marker and a reverse-orientation multiple cloning site.
The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
- Cytomegalovirus (CMV) enhancer-promoter for high-level expression
- Large multiple cloning site in either forward (+) or reverse (-) orientations
- Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
- Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
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