PX386载体_克隆基因_克隆与表达_生化试剂库_商城

PX386载体

产品价格: ￥1000.00

最小起订量：暂无可售数量：999盒

发货时限：: 暂无

所在地区：: 中国上海

有效期至：: 长期有效

最后更新：: 2021-05-13 01:30:02

浏览次数：: 31

货号：	ZY68323
供应商：	泽叶生物
数量：	20
英文名：	PX386
保质期：	三年
保存条件：	-20℃低温保存

载体基本信息

出品公司:	ZYbscience
载体名称:	PX386 或 PX386 Lactobacillus farciminis Cas9
质粒类型:	CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体
高拷贝/低拷贝:	高拷贝
插入位点:	限制性内切酶，多克隆位点
启动子:	CMV
载体大小:	10574 bp
5' 测序引物及序列:	CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列:	C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
载体标签:	NLS (C ter)；HA、NLS (N ter)
载体抗性:	氨苄青霉素
筛选标记:	Neomycin
克隆菌株:	Stbl3
宿主细胞（系）:	昆虫细胞
备注:	--
产品目录号:	ZY68323
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	杆状病毒

载体质粒图谱和多克隆位点信息

PX386载体图谱

载体简介

载体序列

LOCUS PX386 Lactobacillus farciminis Cas9	10574 bp 	DNA	SYNDEFINITION PX386 Lactobacillus farciminis Cas9ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..10574 /organism="PX386 Lactobacillus farciminis Cas9" /mol_type="other DNA" misc_feature 47..1001 /label="Ac_5_flank" CDS complement(343..948) /label="ORF frame 3" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1023..1587 /label="CMV_immearly_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1090..1377 /label="CAG_enhancer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1544..1564 /label="CMV_fwd_primer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1615..1633 /label="T7_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1633..1656 /label="lacO" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1772..1796 /label="attB1" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" CDS 1822..5265 /label="ORF frame 1" /translation="MYPYDVPDYASPKKKRKVEASMADRISLGLDIGVASVGFSVLDI DKGKVIELGARLFSATVAAGNQDRRDMRGARRLLNRNKQRRQDTGKLFKKFGLIDDFD KGSFYDNFNQNLNPYELRVKGLTEQLTKEELAESLYQIVKHRGISYALKDADVDEGGT DYSVSLKINSQELAEKTPAQIQLQRLNDYGKVRGQVVIGDDPDNQKVLLNVFPTSAYE KEAKQIIATQQQFYPESLTDKFTEEYCQILTRKRDYFVGPGNEKSRTDYGIYKTDGRT LDNLFEELIGHDKIYPEELRASAASYTAQLFNVLNDLNNLRILNYEDGKLTKEDKEKI IAEIKNNTTTINMLNVIKKVAGCSKDDIKGFRVNEKDKPEISSMPVYRKIHKDLLKAG VDISDWPVEFIDELSFILTLNTENGEIRKQLNNRLAPKFDFLNADLIQLIIDNKDSFE IKTNNKWHRFSVKTMNKLIPEMMERPVEQMTLLNEMGLVKKDKKRFENNKYLPYKEIA KDIFNPVASKSVREALKIVNAVLKKYGHIDYLVVEMPRDKNLKEEQDNIKEFQNKNKK AKDAAFEAFVKSVGSEQRVKEALSKNRKLQMKMRLWYQQQEIDPYNGKTIDATDLINN PDKFEIDHIIPQSISFYDSINNKTLCFASMNQVKGQKTPYEFMLEGHGQSYDKFKATV MANKNFGKAKRANYLFEENVSDIETRKRFLSRNLVDTRYSSRVVLNSLQDFFREKSAD TKVTVIRGKFTSNMRKHWHIDKTRETFHHHAIDASIIAATPFLRMWKKGGTIFPVKVG EESIDIETGEILDDKNFDKAMYEEPYSGFVSEIMNADDRIKFSHQVDKKMNRKVSDAT IYSTRTGKLAKDKKDAEYIVAKVKDIYSVDGFKKFKKVYDKDKTKFLLYKYDPRTFSK LERIISDCPDKVEKVQTNGKVKAVDISPFEMYRRDHGMIKKYSKKGNGPAIKQLKYLD KKLGSHIDITPANANGKHVILQSLKPWRTDVYLNHETGEYEIMGIKYSDLKFNKNEGY GIKKDKYLEIKKVEEVSEKSEFMFSLYRKDRVKVQDMKTGESVELLFWSRNFSNKKYA ELKPISQAENDKKLPVYGKGRLIKRLIPKNCKIWKVNTTILGDPYYLEKESDSPKDIL D*" misc_feature 1825..1851 /label="HA" /translation="MYPYDVPDYASPKKKRKVEASMADRISLGLDIGVASVGFSVLDI DKGKVIELGARLFSATVAAGNQDRRDMRGARRLLNRNKQRRQDTGKLFKKFGLIDDFD KGSFYDNFNQNLNPYELRVKGLTEQLTKEELAESLYQIVKHRGISYALKDADVDEGGT DYSVSLKINSQELAEKTPAQIQLQRLNDYGKVRGQVVIGDDPDNQKVLLNVFPTSAYE KEAKQIIATQQQFYPESLTDKFTEEYCQILTRKRDYFVGPGNEKSRTDYGIYKTDGRT LDNLFEELIGHDKIYPEELRASAASYTAQLFNVLNDLNNLRILNYEDGKLTKEDKEKI IAEIKNNTTTINMLNVIKKVAGCSKDDIKGFRVNEKDKPEISSMPVYRKIHKDLLKAG VDISDWPVEFIDELSFILTLNTENGEIRKQLNNRLAPKFDFLNADLIQLIIDNKDSFE IKTNNKWHRFSVKTMNKLIPEMMERPVEQMTLLNEMGLVKKDKKRFENNKYLPYKEIA KDIFNPVASKSVREALKIVNAVLKKYGHIDYLVVEMPRDKNLKEEQDNIKEFQNKNKK AKDAAFEAFVKSVGSEQRVKEALSKNRKLQMKMRLWYQQQEIDPYNGKTIDATDLINN PDKFEIDHIIPQSISFYDSINNKTLCFASMNQVKGQKTPYEFMLEGHGQSYDKFKATV MANKNFGKAKRANYLFEENVSDIETRKRFLSRNLVDTRYSSRVVLNSLQDFFREKSAD TKVTVIRGKFTSNMRKHWHIDKTRETFHHHAIDASIIAATPFLRMWKKGGTIFPVKVG EESIDIETGEILDDKNFDKAMYEEPYSGFVSEIMNADDRIKFSHQVDKKMNRKVSDAT IYSTRTGKLAKDKKDAEYIVAKVKDIYSVDGFKKFKKVYDKDKTKFLLYKYDPRTFSK LERIISDCPDKVEKVQTNGKVKAVDISPFEMYRRDHGMIKKYSKKGNGPAIKQLKYLD KKLGSHIDITPANANGKHVILQSLKPWRTDVYLNHETGEYEIMGIKYSDLKFNKNEGY GIKKDKYLEIKKVEEVSEKSEFMFSLYRKDRVKVQDMKTGESVELLFWSRNFSNKKYA ELKPISQAENDKKLPVYGKGRLIKRLIPKNCKIWKVNTTILGDPYYLEKESDSPKDIL D*" misc_feature 1855..1875 /label="SV40 NLS" /translation="MYPYDVPDYASPKKKRKVEASMADRISLGLDIGVASVGFSVLDI DKGKVIELGARLFSATVAAGNQDRRDMRGARRLLNRNKQRRQDTGKLFKKFGLIDDFD KGSFYDNFNQNLNPYELRVKGLTEQLTKEELAESLYQIVKHRGISYALKDADVDEGGT DYSVSLKINSQELAEKTPAQIQLQRLNDYGKVRGQVVIGDDPDNQKVLLNVFPTSAYE KEAKQIIATQQQFYPESLTDKFTEEYCQILTRKRDYFVGPGNEKSRTDYGIYKTDGRT LDNLFEELIGHDKIYPEELRASAASYTAQLFNVLNDLNNLRILNYEDGKLTKEDKEKI IAEIKNNTTTINMLNVIKKVAGCSKDDIKGFRVNEKDKPEISSMPVYRKIHKDLLKAG VDISDWPVEFIDELSFILTLNTENGEIRKQLNNRLAPKFDFLNADLIQLIIDNKDSFE IKTNNKWHRFSVKTMNKLIPEMMERPVEQMTLLNEMGLVKKDKKRFENNKYLPYKEIA KDIFNPVASKSVREALKIVNAVLKKYGHIDYLVVEMPRDKNLKEEQDNIKEFQNKNKK AKDAAFEAFVKSVGSEQRVKEALSKNRKLQMKMRLWYQQQEIDPYNGKTIDATDLINN PDKFEIDHIIPQSISFYDSINNKTLCFASMNQVKGQKTPYEFMLEGHGQSYDKFKATV MANKNFGKAKRANYLFEENVSDIETRKRFLSRNLVDTRYSSRVVLNSLQDFFREKSAD TKVTVIRGKFTSNMRKHWHIDKTRETFHHHAIDASIIAATPFLRMWKKGGTIFPVKVG EESIDIETGEILDDKNFDKAMYEEPYSGFVSEIMNADDRIKFSHQVDKKMNRKVSDAT IYSTRTGKLAKDKKDAEYIVAKVKDIYSVDGFKKFKKVYDKDKTKFLLYKYDPRTFSK LERIISDCPDKVEKVQTNGKVKAVDISPFEMYRRDHGMIKKYSKKGNGPAIKQLKYLD KKLGSHIDITPANANGKHVILQSLKPWRTDVYLNHETGEYEIMGIKYSDLKFNKNEGY GIKKDKYLEIKKVEEVSEKSEFMFSLYRKDRVKVQDMKTGESVELLFWSRNFSNKKYA ELKPISQAENDKKLPVYGKGRLIKRLIPKNCKIWKVNTTILGDPYYLEKESDSPKDIL D*" misc_feature 1861..1875 /label="NLS" /translation="MYPYDVPDYASPKKKRKVEASMADRISLGLDIGVASVGFSVLDI DKGKVIELGARLFSATVAAGNQDRRDMRGARRLLNRNKQRRQDTGKLFKKFGLIDDFD KGSFYDNFNQNLNPYELRVKGLTEQLTKEELAESLYQIVKHRGISYALKDADVDEGGT DYSVSLKINSQELAEKTPAQIQLQRLNDYGKVRGQVVIGDDPDNQKVLLNVFPTSAYE KEAKQIIATQQQFYPESLTDKFTEEYCQILTRKRDYFVGPGNEKSRTDYGIYKTDGRT LDNLFEELIGHDKIYPEELRASAASYTAQLFNVLNDLNNLRILNYEDGKLTKEDKEKI IAEIKNNTTTINMLNVIKKVAGCSKDDIKGFRVNEKDKPEISSMPVYRKIHKDLLKAG VDISDWPVEFIDELSFILTLNTENGEIRKQLNNRLAPKFDFLNADLIQLIIDNKDSFE IKTNNKWHRFSVKTMNKLIPEMMERPVEQMTLLNEMGLVKKDKKRFENNKYLPYKEIA KDIFNPVASKSVREALKIVNAVLKKYGHIDYLVVEMPRDKNLKEEQDNIKEFQNKNKK AKDAAFEAFVKSVGSEQRVKEALSKNRKLQMKMRLWYQQQEIDPYNGKTIDATDLINN PDKFEIDHIIPQSISFYDSINNKTLCFASMNQVKGQKTPYEFMLEGHGQSYDKFKATV MANKNFGKAKRANYLFEENVSDIETRKRFLSRNLVDTRYSSRVVLNSLQDFFREKSAD TKVTVIRGKFTSNMRKHWHIDKTRETFHHHAIDASIIAATPFLRMWKKGGTIFPVKVG EESIDIETGEILDDKNFDKAMYEEPYSGFVSEIMNADDRIKFSHQVDKKMNRKVSDAT IYSTRTGKLAKDKKDAEYIVAKVKDIYSVDGFKKFKKVYDKDKTKFLLYKYDPRTFSK LERIISDCPDKVEKVQTNGKVKAVDISPFEMYRRDHGMIKKYSKKGNGPAIKQLKYLD KKLGSHIDITPANANGKHVILQSLKPWRTDVYLNHETGEYEIMGIKYSDLKFNKNEGY GIKKDKYLEIKKVEEVSEKSEFMFSLYRKDRVKVQDMKTGESVELLFWSRNFSNKKYA ELKPISQAENDKKLPVYGKGRLIKRLIPKNCKIWKVNTTILGDPYYLEKESDSPKDIL D*" CDS complement(2464..2976) /label="ORF frame 3" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" misc_feature complement(5298..5315) /label="Sp6_primer" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" misc_feature 5320..5344 /label="attB2" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" terminator 5356..5822 /label="hGH_PA_terminator" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" terminator 5828..5863 /label="T7_terminator" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" rep_origin 5980..6286 /label="f1_origin" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" misc_feature complement(6400..6420) /label="pBABE_3_primer" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" misc_feature complement(6406..6621) /label="SV40_enhancer" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" promoter 6418..6686 /label="SV40_promoter" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" rep_origin 6585..6662 /label="SV40_origin" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" misc_feature 6647..6666 /label="SV40pro_F_primer" /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG NDLLCFFLVS*" CDS 6801..7595 /label="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 6804..7592 /label="NeoR/KanR" /gene="NeoR/KanR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" CDS complement(7110..7646) /label="ORF frame 1" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 7772..7891 /label="SV40_PA_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 7860..7879 /label="EBV_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 8082..8117 /label="T7_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature complement(8140..8157) /label="Baculovirs_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 8147..8224 /label="3_AcPH" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" CDS complement(8254..8871) /label="ORF frame 3" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" misc_feature 8851..8884 /label="loxP" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" gene complement(9652..10512) /label="Ampicillin" /gene="Ampicillin" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" CDS complement(9652..10512) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN

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