pBMTB-5载体

LOCUS DQ058728 4406 bp DNA circular SYN 08-MAY-2006DEFINITION Broad host range vector pBMTB-5, complete sequence.ACCESSION DQ058728 AUTHORS Lynch,M.D. and Gill,R.T. TITLE Broad host range vectors for stable genomic library construction JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering, University of Colorado, ECCH 111, Campus Box 424, Boulder, CO 80309, USAFEATURES Location/Qualifiers source 1..4406 /organism="Broad host range vector pBMTB-5" /mol_type="other DNA" /db_xref="taxon:334960" regulatory 60..96 /regulatory_class="terminator" /note="SoxR terminator" gene complement(104..1123) /gene="mob" /note="mob gene from pBBR1MCS, GenBank Accession Number U02374" CDS complement(104..1123) /gene="mob" /note="required for mobilization" /codon_start=1 /transl_table=11 /product="Mob" /protein_id="AAY99713.1" /db_xref="GI:69048296" /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK KPLLS" gene 2117..2779 /gene="rep" /note="rep gene from pBBR1, GenBank Accession Number X66730" CDS 2117..2779 /gene="rep" /note="required for replication" /codon_start=1 /transl_table=11 /product="Rep" /protein_id="AAY99714.1" /db_xref="GI:69048297" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP RR" gene 2876..3112 /gene="dhfrIIb" /note="dhfrIIb gene from R388" CDS 2876..3112 /gene="dhfrIIb" /codon_start=1 /transl_table=11 /product="dihydrofolate reductase" /protein_id="AAY99715.1" /db_xref="GI:69048298" /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVG WYCTKLTPEGYAVESESHPGSVQIYPVAALERVA" regulatory 3129..3160 /regulatory_class="terminator" /note="TonB terminator" gene complement(3188..4117) /gene="araC" CDS complement(3188..4117) /gene="araC" /note="regulator" /codon_start=1 /transl_table=11 /product="AraC" /protein_id="AAY99716.1" /db_xref="GI:69048299" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS" regulatory 4125..4392 /regulatory_class="promoter" /note="pBAD promoter"ORIGIN 1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt 121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct 181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct 241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa 301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc 361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt 421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt 481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt 541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct 601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct 661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct 721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg 781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga 841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt 901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc 961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt 1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca 1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt 1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg 1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc 1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc 1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt 1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag 1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc 1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc 1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg 1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc 1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt 1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc 1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg 1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa 1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc 1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt 2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta 2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa 2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc 2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa 2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt 2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg 2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga 2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga 2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca 2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc 2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc 2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt 2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc 2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatggg 2881 tcaaagtagc gatgaagcca acgctcccgt tgcagggcag tttgcgcttc ccctgagtgc 2941 cacctttggc ttaggggatc gcgtacgcaa gaaatctggt gccgcttggc agggtcaagt 3001 cgtcggttgg tattgcacaa aactcactcc tgaaggctat gcggtcgagt ccgaatccca 3061 cccaggctca gtgcaaattt atcctgtggc tgcacttgaa cgtgtggcct aagaatggat 3121 ccccctcaag tcaaaagcct ccggtcggag gcttttgact ttctgctatg gaggtcaggt 3181 atgattttta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga 3241 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt 3301 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct 3361 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc 3421 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat 3481 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat 3541 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa 3601 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt 3661 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat 3721 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa 3781 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg 3841 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca 3901 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga 3961 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat 4021 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc 4081 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct 4141 tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac 4201 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa 4261 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca 4321 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc 4381 cgtttttttg ggctagagaa ttcgtc