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人OP酶联免疫检测试剂| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pAdeasy-1, pAdeasy1 |
| GenBank序列号: | AY370909 |
| 质粒类型: | 腺病毒表达载体，哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 33441 bp |
| 5' 测序引物及序列: | / |
| 3' 测序引物及序列: | / |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 备注: | -- |
| 产品目录号: | ZY240005 |
| 稳定性: | / |
| 组成型: | -- |
| 病毒/非病毒: | 腺病毒 |
| 货号： | ZY |
| 供应商： | 泽叶生物 |
| 数量： | 20 |
| 英文名： | pAdEasy-1 |
| 保质期： | 三年 |
| 保存条件： | -20℃低温保存 |
pAdEasy1载体基本信息
pAdEasy1载体质粒图谱和多克隆位点信息
pAdEasy1载体简介
The plasmid pAdEasy-1, containing most of the human adenovirus serotype 5 (Ad5) genome, is deleted for the genes E1 and E3. The removal of these two viral genes creates space for foreign DNA and eliminates selfreplication capabilities. The E1 deletion renders the viruses defective for replication and incapable of producing infectious viral particles in target cells (provided there is no complementation by the host cell); the E3 region encodes proteins involved in evading host immunity and is dispensable. The deletion of both genes creates room for up to 7.5 kb of foreign DNA that can be inserted into the Ad5 genome. The E1 gene, which is necessary for production of viral particles, is provided in trans by AD-293 cells. pAdEasy-1 carries the ampicillin resistance gene, which is lost after recombination with a shuttle vector.
温馨提示：不可用于临床治疗。
