pMHE3载体

LOCUS AY299694 6290 bp DNA circular SYN 10-FEB-2004DEFINITION Broad host range expression vector pMHE3, complete sequence.ACCESSION AY299694VERSION AY299694.1 GI:34334142 TITLE Modular broad-host-range expression vectors for single-protein and protein complex purification JOURNAL Appl. Environ. Microbiol. 70 (2), 712-721 (2004) PUBMED 14766546 AUTHORS Fodor,B.D. JOURNAL Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of Sciences, Temesvari krt., Szeged, Csongrad 6701, HungaryFEATURES Location/Qualifiers source 1..6290 /organism="Broad host range expression vector pMHE3" /mol_type="genomic DNA" /db_xref="taxon:244052" primer_bind 111..129 /note="T7 promoter primer" regulatory 111..127 /regulatory_class="promoter" /note="T7 promoter" misc_feature 128..153 /note="lac operator" regulatory 184..190 /regulatory_class="ribosome_binding_site" misc_feature 199..231 /note="T7-tag" misc_feature 280..297 /note="His-tag" primer_bind complement(350..367) /note="T7 terminator primer" regulatory 363..410 /regulatory_class="terminator" /note="T7 terminator" rep_origin 1436..2098 /note="pBBR1 oriV" misc_feature 3092..4085 /note="RP4 mob; oriT" regulatory complement(4175..4290) /regulatory_class="terminator" /note="T4 terminator" gene complement(4550..5341) /gene="aadA" CDS complement(4550..5341) /gene="aadA" /note="streptomycin resistance" /codon_start=1 /transl_table=11 /product="aminoglycoside-3:adenyltransferase fusion" /protein_id="AAQ64665.1" /db_xref="GI:34334143" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" regulatory 6077..6192 /regulatory_class="terminator" /note="T4 terminator"ORIGIN 1 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc 61 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact 121 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt 181 taagaaggag atatacatat ggctagcatg actggtggac agcaaatggg tcgggatccg 241 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga 301 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa 361 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga 421 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg 481 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 541 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 601 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 661 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 721 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 781 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 841 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatattt tgttaaaatt 901 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgact gcgatgagtg 961 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac 1021 gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg 1081 tcgtcggttc agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt 1141 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc 1201 aggctctccc cgtggaggta ataattgacg atatgatcat ttattctgcc tcccagagcc 1261 tgataaaaac ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc 1321 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgaggcg 1381 gctacagccg atagtctgga acagcgcact tacgggttgc tgcgcaaccc aagtgctacc 1441 ggcgcggcag cgtgacccgt gtcggcggct ccaacggctc gccatcgtcc agaaaacacg 1501 gctcatcggg catcggcagg cgctgctgcc cgcgccgttc ccattcctcc gtttcggtca 1561 aggctggcag gtctggttcc atgcccggaa tgccgggctg gctgggcggc tcctcgccgg 1621 ggccggtcgg tagttgctgc tcgcccggat acagggtcgg gatgcggcgc aggtcgccat 1681 gccccaacag cgattcgtcc tggtcgtcgt gatcaaccac cacggcggca ctgaacaccg 1741 acaggcgcaa ctggtcgcgg ggctggcccc acgccacgcg gtcattgacc acgtaggccg 1801 acacggtgcc ggggccgttg agcttcacga cggagatcca gcgctcggcc accaagtcct 1861 tgactgcgta ttggaccgtc cgcaaagaac gtccgatgag cttggaaagt gtcttctggc 1921 tgaccaccac ggcgttctgg tggcccatct gcgccacgag gtgatgcagc agcattgccg 1981 ccgtgggttt cctcgcaata agcccggccc acgcctcatg cgctttgcgt tccgtttgca 2041 cccagtgacc gggcttgttc ttggcttgaa tgccgatttc tctggactgc gtggccatgc 2101 ttatctccat gcggtagggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt 2161 ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct 2221 ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt 2281 ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc 2341 caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa 2401 aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg 2461 cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg 2521 cttcgccttc gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca 2581 agccgctgcg cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca 2641 agcgaagcgc gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat 2701 ggggcaaggc cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag 2761 ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc 2821 cagcagggtt gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg 2881 tccacatatc cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag 2941 caagcccgta gggcgccgca gccgccgtag gcggtcacga ctttgcgaag caaagtctag 3001 tgagtatact caagcattga gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga 3061 gccggttgga caccaaaagg gaggggcagg catggcggca tacgcgatca tgcgatgcaa 3121 gaagctggcg aaaatgggca acgtggcggc cagtctcaag cacgcctacc gcgagcgcga 3181 gacgcccaac gctgacgcca gcaggacgcc agagaacgag cactgggcgg ccagcagcac 3241 cgatgaagcg atgggccgac tgcgcgagtt gctgccagag aagcggcgca aggacgctgt 3301 gttggcggtc gagtacgtca tgacggccag cccggaatgg tggaagtcgg ccagccaaga 3361 acagcaggcg gcgttcttcg agaaggcgca caagtggctg gcggacaagt acggggcgga 3421 tcgcatcgtg acggccagca tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt 3481 ggtgccgctg acgcaggacg gcaggctgtc ggccaaggag ttcatcggca acaaagcgca 3541 gatgacccgc gaccagacca cgtttgcggc cgctgtggcc gatctagggc tgcaacgggg 3601 catcgagggc agcaaggcac gtcacacgcg cattcaggcg ttctacgagg ccctggagcg 3661 gccaccagtg ggccacgtca ccatcagccc gcaagcggtc gagccacgcg cctatgcacc 3721 gcagggattg gccgaaaagc tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc 3781 cgaccggctg acaaaagcgg ttcggcaggg gtatgagcct gccctacagg ccgccgcagg 3841 agcgcgtgag atgcgcaaga aggccgatca agcccaagag acggcccgag accttcggga 3901 gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc aggccaaggc 3961 cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg aagtccagcg 4021 ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg aaaagtgcca 4081 cctggcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt 4141 gaacgaattg ttaggtggcg gtacttgggt cgatagcttt atgcttgtaa accgttttgt 4201 gaaaaaattt ttaaaataaa aaaggggacc tctagggtcc ccaattaatt agtaatataa 4261 tctattaaag gtcattcaaa aggtcatcca ccggatcagc ttagtaaagc cctcgctaga 4321 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc 4381 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa 4441 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg 4501 agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat tgttagacat tatttgccga 4561 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg 4621 aggccaagcg atcttcttct tgtccaagat aagcctgtct agctt