pMHE5载体

LOCUS AY299696 5938 bp DNA circular SYN 10-FEB-2004DEFINITION Broad host range expression vector pMHE5, complete sequence.ACCESSION AY299696VERSION AY299696.1 GI:34334146 TITLE Modular broad-host-range expression vectors for single-protein and protein complex purification JOURNAL Appl. Environ. Microbiol. 70 (2), 712-721 (2004) PUBMED 14766546 AUTHORS Fodor,B.D. TITLE Direct Submission JOURNAL Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of Sciences, Temesvari krt., Szeged, Csongrad 6701, HungaryFEATURES Location/Qualifiers source 1..5938 /organism="Broad host range expression vector pMHE5" /mol_type="genomic DNA" /db_xref="taxon:244054" primer_bind 193..211 /note="T7 promoter primer" regulatory 193..209 /regulatory_class="promoter" /note="T7 promoter" misc_feature 210..235 /note="lac operator" regulatory 266..272 /regulatory_class="ribosome_binding_site" misc_feature 280..282 /note="fusion protein start codon" misc_feature 286..303 /note="His-tag" misc_feature 304..315 /note="Factor Xa cleavage site" misc_feature 370..393 /note="FLAG-tag" misc_feature 379..393 /note="enterokinase cleavage site" misc_feature 397..420 /note="Strep-tag II" primer_bind complement(474..491) /note="T7 terminator primer" regulatory 487..534 /regulatory_class="terminator" /note="T7 terminator" rep_origin 1166..1828 /note="pBBR1 oriV" misc_feature 2822..3815 /note="RP4 mob; oriT" regulatory complement(3905..4020) /regulatory_class="terminator" /note="T4 terminator" gene complement(4280..5071) /gene="aadA" CDS complement(4280..5071) /gene="aadA" /note="streptomycin resistance" /codon_start=1 /transl_table=11 /product="aminoglycoside-3:adenyltransferase fusion" /protein_id="AAQ64667.1" /db_xref="GI:34334147" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" regulatory 5807..5922 /regulatory_class="terminator" /note="T4 terminator"ORIGIN 1 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc 61 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc 121 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg 181 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc 241 tagaaataat tttgtttaac tttaagaagg agatatacca tggggcatca tcatcatcat 301 catatcgagg gaaggcctgt cgacggtatc gataagcttg atatcgaatt cctgcagctc 361 gagggatccg actacaagga cgacgacgac aagaactgga gccatccgca gttcgagaag 421 tgaagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga 481 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa 541 aggaggaact atatccggat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga 601 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 661 ttttaaccaa taggccgact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag 721 ttattggtgc ccttaaacgc ctggtgctac gcctgaataa gtgataataa gcggatgaat 781 ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag 841 ccgcttatgt ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt 901 gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgacg 961 atatgatcat ttattctgcc tcccagagcc tgataaaaac ggtgaatccg ttagcgaggt 1021 gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg 1081 gaggcagaca aggtataggg cggcgaggcg gctacagccg atagtctgga acagcgcact 1141 tacgggttgc tgcgcaaccc aagtgctacc ggcgcggcag cgtgacccgt gtcggcggct 1201 ccaacggctc gccatcgtcc agaaaacacg gctcatcggg catcggcagg cgctgctgcc 1261 cgcgccgttc ccattcctcc gtttcggtca aggctggcag gtctggttcc atgcccggaa 1321 tgccgggctg gctgggcggc tcctcgccgg ggccggtcgg tagttgctgc tcgcccggat 1381 acagggtcgg gatgcggcgc aggtcgccat gccccaacag cgattcgtcc tggtcgtcgt 1441 gatcaaccac cacggcggca ctgaacaccg acaggcgcaa ctggtcgcgg ggctggcccc 1501 acgccacgcg gtcattgacc acgtaggccg acacggtgcc ggggccgttg agcttcacga 1561 cggagatcca gcgctcggcc accaagtcct tgactgcgta ttggaccgtc cgcaaagaac 1621 gtccgatgag cttggaaagt gtcttctggc tgaccaccac ggcgttctgg tggcccatct 1681 gcgccacgag gtgatgcagc agcattgccg ccgtgggttt cctcgcaata agcccggccc 1741 acgcctcatg cgctttgcgt tccgtttgca cccagtgacc gggcttgttc ttggcttgaa 1801 tgccgatttc tctggactgc gtggccatgc ttatctccat gcggtagggt gccgcacggt 1861 tgcggcacca tgcgcaatca gctgcaactt ttcggcagcg cgacaacaat tatgcgttgc 1921 gtaaaagtgg cagtcaatta cagattttct ttaacctacg caatgagcta ttgcgggggg 1981 tgccgcaatg agctgttgcg tacccccctt ttttaagttg ttgattttta agtctttcgc 2041 atttcgccct atatctagtt ctttggtgcc caaagaaggg cacccctgcg gggttccccc 2101 acgccttcgg cgcggctccc cctccggcaa aaagtggccc ctccggggct tgttgatcga 2161 ctgcgcggcc ttcggccttg cccaaggtgg cgctgccccc ttggaacccc cgcactcgcc 2221 gccgtgaggc tcggggggca ggcgggcggg cttcgccttc gactgccccc actcgcatag 2281 gcttgggtcg ttccaggcgc gtcaaggcca agccgctgcg cggtcgctgc gcgagccttg 2341 acccgccttc cacttggtgt ccaaccggca agcgaagcgc gcaggccgca ggccggaggc 2401 ttttccccag agaaaattaa aaaaattgat ggggcaaggc cgcaggccgc gcagttggag 2461 ccggtgggta tgtggtcgaa ggctgggtag ccggtgggca atccctgtgg tcaagctcgt 2521 gggcaggcgc agcctgtcca tcagcttgtc cagcagggtt gtccacgggc cgagcgaagc 2581 gagccagccg gtggccgctc gcggccatcg tccacatatc cacgggctgg caagggagcg 2641 cagcgaccgc gcagggcgaa gcccggagag caagcccgta gggcgccgca gccgccgtag 2701 gcggtcacga ctttgcgaag caaagtctag tgagtatact caagcattga gtggcccgcc 2761 ggaggcaccg ccttgcgctg cccccgtcga gccggttgga caccaaaagg gaggggcagg 2821 catggcggca tacgcgatca tgcgatgcaa gaagctggcg aaaatgggca acgtggcggc 2881 cagtctcaag cacgcctacc gcgagcgcga gacgcccaac gctgacgcca gcaggacgcc 2941 agagaacgag cactgggcgg ccagcagcac cgatgaagcg atgggccgac tgcgcgagtt 3001 gctgccagag aagcggcgca aggacgctgt gttggcggtc gagtacgtca tgacggccag 3061 cccggaatgg tggaagtcgg ccagccaaga acagcaggcg gcgttcttcg agaaggcgca 3121 caagtggctg gcggacaagt acggggcgga tcgcatcgtg acggccagca tccaccgtga 3181 cgaaaccagc ccgcacatga ccgcgttcgt ggtgccgctg acgcaggacg gcaggctgtc 3241 ggccaaggag ttcatcggca acaaagcgca gatgacccgc gaccagacca cgtttgcggc 3301 cgctgtggcc gatctagggc tgcaacgggg catcgagggc agcaaggcac gtcacacgcg 3361 cattcaggcg ttctacgagg ccctggagcg gccaccagtg ggccacgtca ccatcagccc 3421 gcaagcggtc gagccacgcg cctatgcacc gcagggattg gccgaaaagc tgggaatctc 3481 aaagcgcgtt gagacgccgg aagccgtggc cgaccggctg acaaaagcgg ttcggcaggg 3541 gtatgagcct gccctacagg ccgccgcagg agcgcgtgag atgcgcaaga aggccgatca 3601 agcccaagag acggcccgag accttcggga gcgcctgaag cccgttctgg acgccctggg 3661 gccgttgaat cgggatatgc aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa 3721 gctgctgacg gaacagcggg aagtccagcg ccagaaacag gcccagcgcc agcaggaacg 3781 cgggcgcgca catttccccg aaaagtgcca cctggcggcg ttgtgacaat ttaccgaaca 3841 actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt 3901 cgatagcttt atgcttgtaa accgttttgt gaaaaaattt ttaaaataaa aaaggggacc 3961 tctagggtcc ccaattaatt agtaatataa tctattaaag gtcattcaaa aggtcatcca 4021 ccggatcagc ttagtaaagc cctcgctaga ttttaatgcg gatgttgcga ttacttcgcc 4081 aactattgcg ataacaagaa aaagccagcc tttcatgata tatctcccaa tttgtgtagg 4141 gcttattatg cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag 4201 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc 4261 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt 4321 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat 4381 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc 4441 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg 4501 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg 4561 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct 4621 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca 4681 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt 4741 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa 4801 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca 4861 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca 4921 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta 4981 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag 5041 tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctttgtttta gggcgactgc 5101 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 5161 cttgctgctt ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca 5221 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc 5281 gtgagcgcat acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg 5341 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag 5401 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc 5461 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgccctggc 5521 ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg 5581 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc cagcttctgt 5641 atggaacggg catgcggatc agtgagggtt tgcaactgcg ggtcaaggat ctggatttcg 5701 atcacggcac gatcatcgtg cgggagggca agggctccaa ggatcgggcc ttgatgttac 5761 ccgagagctt ggcacccagc ctgcgcgagc aggggaattg atccggtgga tgaccttttg 5821 aatgaccttt aatagattat attactaatt aattggggac cctagaggtc ccctttttta 5881 ttttaaaaat tttttcacaa aacggtttac aagcataaag ctcaaggaga tggcgccc//