pEarleyGate103

载体基本信息

载体质粒图谱和多克隆位点信息

载体简介

载体序列

LOCUS Exported 12411 bp ds-DNA circular SYN 26-JUL-2016DEFINITION Gateway(R)-compatible plant transformation vector with GFP and 6xHis C-terminal tags.ACCESSION .VERSION .KEYWORDS pEarleyGate 103SOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 12411) AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS. TITLE Gateway-compatible vectors for plant functional genomics and proteomics. JOURNAL Plant J. 2006;45:616-29. PUBMED 16441352REFERENCE 2 (bases 1 to 12411) AUTHORS Pikaard Lab TITLE Direct Submission JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 http://www.snapgene.comCOMMENT The sequence was corrected by inserting a G at position 8519.FEATURES Location/Qualifiers source 1..12411 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" misc_feature 1..25 /note="LB T-DNA repeat" /note="left border repeat from nopaline C58 T-DNA" terminator 111..363 /note="MAS terminator" /note="mannopine synthase terminator" CDS complement(373..924) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase" /note="BlpR" /note="confers resistance to bialophos or phosphinothricin" /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTEI" promoter complement(930..1310) /note="MAS promoter" /note="mannopine synthase promoter (Velten et al., 1984)" promoter 2306..2651 /note="CaMV 35S promoter" /note="strong constitutive promoter from cauliflower mosaic virus" protein_bind 2663..2782 /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR1" /note="recombination site for the Gateway(R) LR reaction" promoter 2812..2842 /note="lac UV5 promoter" /note="E. coli lac promoter with an ""up"" mutation" CDS 2896..3555 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" CDS 3897..4202 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /note="ccdB" /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" protein_bind complement(4243..4367) /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR2" /note="recombination site for the Gateway(R) LR reaction" CDS 4390..5100 /codon_start=1 /gene="mgfp5" /product="GFP with folding enhancement mutations" /note="mgfp5" /note="suitable for expression in plants due to removal of a cryptic intron" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" CDS 5107..5124 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" terminator 5182..5889 /note="OCS terminator" /note="octopine synthase terminator" misc_feature 6133..6157 /note="RB T-DNA repeat" /note="right border repeat from nopaline C58 T-DNA" CDS 7457..8086 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /note="pVS1 StaA" /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 8515..9588 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /note="pVS1 RepA" /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 9654..9848 /note="pVS1 oriV" /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 10192..10332 /note="bom" /note="basis of mobility region from pBR322" rep_origin complement(10518..11106) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(11193..11987) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"ORIGIN 1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg 61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat 121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat 181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa 241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc 301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc 361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct 421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg 481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg 541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg 601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga 661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc 721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc 781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat 841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc 901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat 961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt 1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc 1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt 1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga 1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa 1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat 1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg 1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata 1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa 1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac 1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa 1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc 1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt 1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac 1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt 1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga 1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat 1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat 2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt 2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac 2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga 2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata 2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc 2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag 2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg 2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg 2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg 2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt 2641 tggagaggac acgctcgaga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa 2701 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg 2761 taaaacacaa catatccagt catattggcg gccgcattag gcaccccagg ctttacactt 2821 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga 2881 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa 2941 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag 3001 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt 3061 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg 3121 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc 3181 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag 3241 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct 3301 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt 3361 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa 3421 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt 3481 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg 3541 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat 3601 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca 3661 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt 3721 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga 3781 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt 3841 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc 3901 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga 3961 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc 4021 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc 4081 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg 4141 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat 4201 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt 4261 tgtgttttac agcattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata 4321 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgct cgagatggta 4381 gatctgacta gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta 4441 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca 4501 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccgtggcca 4561 acacttgtca ctactttctc ttatggtgtt caatgctttt caagataccc agatcatatg 4621 aagcggcacg acttcttcaa gagcgccatg cctgagggat acgtgcagga gaggaccatc 4681 ttcttcaagg acgacgggaa ctacaagaca cgtgctgaag tcaagtttga gggagacacc 4741 ctcgtcaaca ggatcgagct taagggaatc gatttcaagg aggacggaaa catcctcggc 4801 cacaagttgg aatacaacta caactcccac aacgtataca tcatggccga caagcaaaag 4861 aacggcatca aagccaactt caagacccgc cacaacatcg aagacggcgg cgtgcaactc 4921 gctgatcatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac 4981 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg 5041 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa 5101 gctagccacc accaccacca ccacgtgtga cctaggtgag tctagagagt taattaagac 5161 ccgggactag tccctagagt cctgctttaa tgagatatgc gagacgccta tgatcgcatg 5221 atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat 5281 ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta 5341 tgaataatat tctccgttca atttactgat tgtaccctac tacttatatg tacaatatta 5401 aaatgaaaac aatatattgt gctgaatagg tttatagcga catctatgat agagcgccac 5461 aataacaaac aattgcgttt tattattaca aatccaattt taaaaaaagc ggcagaaccg 5521 gtcaaaccta aaagactgat tacataaatc ttattcaaat ttcaaaagtg ccccaggggc 5581 tagtatctac gacacaccga gcggcgaact aataacgctc actgaaggga actccggttc 5641 cccgccggcg cgcatgggtg agattccttg aagttgagta ttggccgtcc gctctaccga 5701 aagttacggg caccattcaa cccggtccag cacggcggcc gggtaaccga cttgctgccc 5761 cgagaattat gcagcatttt tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc 5821 ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag 5881 gctcagcagg acctgcaggc atgcaagctt ggcactggcc gtcgttttac aacgtcgtga 5941 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 6001 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 6061 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa 6121 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta 6181 gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca 6241 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat 6301 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag 6361 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt 6421 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag 6481 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc 6541 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc 6601 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac 6661 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt 6721 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac 6781 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag 6841 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg 6901 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc 6961 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc 7021 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt 7081 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc 7141 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg 7201 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct 7261 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc 7321 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg 7381 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa 7441 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa 7501 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt 7561 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc 7621 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg 7681 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat 7741 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac 7801 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc 7861 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc 7921 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc 7981 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg 8041 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa 8101 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc 8161 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag 8221 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt 8281 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat 8341 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac 8401 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg 8461 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc 8521 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag 8581 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt 8641 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc 8701 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg 8761 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt 8821 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac 8881 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg 8941 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag 9001 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat 9061 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt 9121 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa 9181 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag 9241 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg 9301 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg 9361 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc 9421 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca 9481 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc 9541 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag 9601 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat 9661 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac 9721 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa 9781 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc 9841 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg 9901 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca 9961 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca 10021 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga 10081 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 10141 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat 10201 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag 10261 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 10321 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 10381 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 10441 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 10501 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 10561 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 10621 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 10681 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 10741 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 10801 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 10861 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 10921 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 10981 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 11041 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 11101 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 11161 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa 11221 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac 11281 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg 11341 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag 11401 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc 11461 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg 11521 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct 11581 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc 11641 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg 11701 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg 11761 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca 11821 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct 11881 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat 11941 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt 12001 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca 12061 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt 12121 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac 12181 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg 12241 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt 12301 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc 12361 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg g//