货号: | P00020 |
供应商: | 江蓝纯 |
数量: | 99 |
规格: | 10ug/50ug/500ug/1mg |
Known as | Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2 |
Derived from | E.coli |
Purity | ED50 is less than 0.1 ng/ml. Specific Activity of 1.0 x 107 IU/ mg.measured by the dose-dependent stimulation of murine CTLL-2 cells.Greater than 95% as determined by reducing SDS-PAGE. |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0. |
Shipping | The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 4 months |
Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in 2X PBS.Please aliquot the reconstituted solution to minimize freeze-t cycles. |
Endotoxin | Less than 0.1 ng/ug (1 IEU/ug). |
Amino Acid Sequence | MPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLI SNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT |
Background | Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native IL-2. |
Recombinant Human Interleukin-2 is produced by our E.coli expression system and the target gene encoding
Pro22-Thr153 is expressed.
温馨提示:不可用于临床治疗。
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