货号: | OADC00002 |
抗体英文名: | Methylcholanthrene Antibody (OADC00002) |
应用范围: | DB |
宿主: | Rabbit |
适应物种: | Human |
标记物: | Unconjugated |
克隆性: | 否 |
保存条件: | Store at -20C. Avoid repeated freeze/thaw cycles. |
形态: | Liquid. Provided in PBS containing 0.05% azide and 0.05% ProClin 300. |
规格: | 50 ug |
3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OADC00002
3-mCr
Dot blot analysis using the Aviva antibody directed against 3-mCr. To demonstrate the specificity of the Aviva antibody against 3-mC, a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo's were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skim milk and 1% BSA. Figure shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.
温馨提示:不可用于临床治疗。
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