Mouse Complement fragment 4a (C4a) ELISA Kit

货号：	AE59223MO
样本：	Serum, Plasma, Other biological fluids
适应物种：	Mouse (Mus musculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	13.5 pg/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：C4a
别名：N/A
检测范围：31.25-2000 pg/mL
产品概述：Complement C4-A is the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene.
特异性：This assay has high sensitivity and excellent specificity for detection of Mouse C4a. No significant cross-reactivity or interference between Mouse C4a and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Mouse C4a and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse C4a in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse C4a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate C4a in samples. An antibody specific for C4a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC4a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4a bound in the initial step. The color development is stopped and the intensity of the color is measured.