Mouse Kallikrein-1 (KLK1) ELISA Kit

货号：	AE36495MO
样本：	Serum, Plasma, Other biological fluids
标记物：	P15947
适应物种：	Mouse (Mus musculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	19.1 pg/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：KLK1
别名：KLKR; Klk6; hK1; glandular kallikrein 1|kallikrein 1; renal/pancreas/salivary|kallikrein serine protease 1|tissue kallikrein
检测范围：49.38-4000 pg/mL
产品概述：KLK1, or tissue kallikrein is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. Kallikrein 1 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. the deduced 262-amino acid KLK1 protein contains a putative signal peptide, followed by a short activating peptide and the protease domain. The protease domain contains the catalytic triad of his65, asp120, and ser214. RT-PCR detected variable expression of KLK1 in most of the 35 tissues examined. Highest expression was in pancreas, salivary gland, thyroid, parotid gland, fetal and adult skin, kidney, and liver.
特异性：This assay has high sensitivity and excellent specificity for detection of Mouse KLK1. No significant cross-reactivity or interference between Mouse KLK1 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Mouse KLK1 and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse KLK1 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse KLK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate KLK1 in samples. An antibody specific for KLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.