货号: | EGM0321 |
抗体名: | TNF-α多克隆抗体 |
抗体英文名: | TNF-α Polyclonal Antibody |
靶点: | TNF-α |
浓度: | 2mg/ml |
应用范围: | WB IHC |
宿主: | 兔 |
适应物种: | Human,Rat,Mouse |
标记物: | 无 |
克隆性: | 否 |
保存条件: | Store at -20℃. Avoid freeze / thaw cycles |
形态: | 液体 |
亚型: | IgG |
免疫原: | Recombinant Human Tumor necrosis factor alpha protein |
规格: | 30ul/60ul/120ul |
2 包邮,不提供发票
Immunogen: | Recombinant Human Tumor necrosis factor alpha protein | ||
Synonym: | APC1,Cachectin,DIF,Macrophage cytotoxic factor,TNF,TNF a,TNF alpha,TNFA,TNF-a,TNFSF2,Tumor necrosis factor | ||
Calculated MW: | 26kD | ||
Observed MW: | 26kD | ||
Source: | Rabbit |
Isotype: | IgG | Reactivity: Human,Rat,Mouse Clonality: Polyclonal | |
Application: | WB,IHC | ||
Purify: | Affinity purification | ||
Concentration: | 2mg/ml | ||
Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | ||
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of Rat kidney and mouse kidney tissue, using TNF-α Polyclonal Antibody at dilution of 1:600 | Immunohistochemistry of paraffin-embedded Rat kidney using TNF-α antibody at dilution of 1:50 |
Protocols: | Buffer-Formulation WB-Guide IHC-Guide IP-Guide IF-Guide | ||
Background: | TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. |