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免疫印迹western实验
myc标签抗体偶联affin
flag标签抗体偶联affi
WB实验外包
GFP标签抗体偶联affin
Western Blot实验代做
免疫印迹Western实验| 货号： | AT0079 |
| 供应商： | 全国独家总代上海维景生物 |
| 英文名： | anti HA affinity gel |
| 规格： | 1ml,10ml |
可来电或是QQ索取详细产品说明书
折扣信息请登录上海维景公司网站 http://www.weijingbiotech.com/cmctag/affinity_gel.html
Agarose beads Conjugated anti-HA Mouse monoclonal antibody
Catalog number：AT0079 Size：1ml
Specificity： Anti-HA Agarose beads(Affinity gel) is a mouse monoclonal antibody that is covalently attached to agarose beads by hydrazide linkage. The antibody binds HA at the N-terminal, C-terminal and internal locations of fusion proteins.
Tested applications: Immunoprecipitation (IP),
40 ml of gel suspension (~20μl of packed beads) for ~1000μg of crude total protein solution.
Optimal dilutions/concentrations should be determined by the end user.
Reactivity: The antibody detects and captures HA-Tagged fusion proteins overexpressed in mammalian cells or E.coli.
Immunogen: This monoclonal antibody is produced by immunizing mouses with a synthetic peptide (KLH-coupled) containing YPYDVPDYA.
Form: The productis supplied as a 50% suspension in 50% glycerol with 10 mM sodium phosphate and 150 mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide
Binding capacity: 30-50 nmoles of HA-tagged fusion protein per 1 ml of settled gel.
Isotype: Mouse IgG1
Recommended IP Lysis buffer for mammalian cells: 50 mM Tris HCl, pH 7.4, with 150 mM NaCl, 1 mM EDTA, and 1% TRITON X-100.
Storage instruction:
The gel is supplied in 50% glycerol, so the gel can be stored at –20 °C for maximum stability. The unopened product is stable for one year when stored as indicated.
For used and recycled gel, the beads should be cleaned and stored in 50% glycerol with TBS or PBS buffer containing 0.02% sodium azide to protect the product. Stored at –20 °C for maximum stability or 4 °C for short term(1-2 weeks). Do not freeze in the absence of glycerol.
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