货号: | AT0083 |
供应商: | 全国独家总代上海维景生物 |
英文名: | anti GFP affinity gel |
规格: | 500ul,1ml |
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Agarose Conjugated anti-GFP (Green Fluorescent Protein) mAb
Catalog number:AT0083 Size:500ul
Background: Since the detection of intracellular Aequorea victoria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. Agarose conjugated anti-GFP monoclonal antibody can detect GFP fusion protein on immunoprecipitation.
Tested applications: Immunoprecipitation (IP), 20μL of gel slurry.
2 μg of GFP can be precipitated with 20 μL of 50% gel slurry. Optimal working dilutions must be determined by end user.
Reactivity: This antibody reacts with GFP fusion protein on Immunoprecipitation. It reacts with EBFP, ECFP, EGFP, Venus and Sapphire.
Immunogen: GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP
Form: anti-GFP monoclonal antibody covalently coupled to agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 500 μL.
*Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials containing azide into drain.
Isotype: Rat IgG2a
Storage instruction: Store at +4°C and do not freeze.
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