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BTBD3
pTRE2pur Vector
山羊抗兔IgG二抗 (HRP)
通过嘉定区市场监管局认证 
免疫印迹western实验
myc标签抗体偶联affin
HA标签抗体偶联affini
WB实验外包
GFP标签抗体偶联affin
Western Blot实验代做
免疫印迹Western实验| 货号： | AT0078 |
| 抗体名： | flag标签抗体偶联affinity gel亲和凝胶 |
| 抗体英文名： | anti flag affinity gel |
| 靶点： | flag |
| 应用范围： | IP,Co-IP |
| 标记物： | agarose beads |
| 克隆性： | 是 |
| 保存条件： | –20 °C |
| 形态： | 液体 |
| 规格： | 1ml,10ml |
可来电或是QQ索取详细产品说明书
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Agarose beads Conjugated anti-FLAG Mouse monoclonal antibody
Catalog number：AT0078 Size：1ml
Specificity： Anti-FLAG Agarose beads(Affinity gel) is a mouse monoclonal antibody that is covalently attached to agarose beads by hydrazide linkage. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins.
Tested applications: Immunoprecipitation (IP),
40 ml of gel suspension (~20μl of packed beads) for ~1000μg of crude total protein solution.
Optimal dilutions/concentrations should be determined by the end user.
Reactivity: The antibody detects and captures DYKDDDDK-Tagged fusion proteins overexpressed in mammalian cells or E.coli.
Immunogen: This monoclonal antibody is produced by immunizing mouses with a synthetic peptide (KLH-coupled) containing DYKDDDDK.
Form: The productis supplied as a 50% suspension in 50% glycerol with 10 mM sodium phosphate and 150 mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide
Binding capacity: >50 nmoles of FLAG fusion protein per 1 ml of settled gel.
Isotype: Mouse IgG1
Recommended IP Lysis buffer for mammalian cells: 50 mM Tris HCl, pH 7.4, with 150 mM NaCl, 1 mM EDTA, and 1% TRITON X-100.
Storage instruction:
The gel is supplied in 50% glycerol, so the gel can be stored at –20 °C for maximum stability. The unopened product is stable for one year when stored as indicated.
For used and recycled gel, the beads should be cleaned and stored in 50% glycerol with TBS or PBS buffer containing 0.02% sodium azide to protect the product. Stored at –20 °C for maximum stability or 4 °C for short term(1-2 weeks). Do not freeze in the absence of glycerol.
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