Human R-spondin-1 (RSPO1) ELISA Kit

货号：	AE21095HU
样本：	Serum, Plasma, Other biological fluids
标记物：	Q2MKA7
适应物种：	Human (Homo sapiens)
应用：	Quantitative
检测方法：	Sandwich
检测限：	5.5 pg/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：RSPO1
别名：RP11-566C13.1; CRISTIN3; FLJ40906; RSPO; OTTHUMP00000180612|OTTHUMP00000180613|R-spondin1|roof plate-specific spondin
检测范围：15.6-1000 pg/mL
产品概述：RSPO1 is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.R-spondin transcripts were observed mainly in central nervous system (CNS) tissues, especially in association with the roof plate structure, in 10- and 12-dpc embryos. Outside the CNS, R-spondin transcripts were detected in forelimb buds after 10 dpc, especially in the body trunk and the proximal posterior part of the anterior limb bud. Expression was also observed in facial parenchyma surrounding the nasal cavity at 12 dpc.
特异性：This assay has high sensitivity and excellent specificity for detection of Human RSPO1. No significant cross-reactivity or interference between Human RSPO1 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Human RSPO1 and the recovery rates were calculated by comparing the measured value to the expected amount of Human RSPO1 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human RSPO1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate RSPO1 in samples. An antibody specific for RSPO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRSPO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RSPO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RSPO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.