Mouse Toll-interacting protein (TOLLIP) ELISA Kit

货号：	AE13839MO
样本：	Serum, Plasma, Other biological fluids
标记物：	Q9QZ06
适应物种：	Mouse (Mus musculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	Request Information
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：TOLLIP
别名：FLJ33531; IL-1RAcPIP; Toll-interacting protein|adapter protein
检测范围：Request Information
产品概述：Toll interacting protein, also known as TOLLIP, is an inhibitory adaptor protein .It is an inhibitory adaptor protein within Toll-like receptors (TLR). The TLR pathway is a part of the innate immune system that recognizes structurally conserved molecular patterns of microbial pathogens, leading to an inflammatory immune response.Compared with the full-length isoform, 2 of the human proteins lack the N-terminal TBD, and 1 lacks the C2 domain. A mouse-specific isoform lacks the C-terminal CUE domain. EST database analysis suggested that full-length human TOLLIP is ubiquitously expressed. Expression of the other human variants appeared to be more restricted, with evidence for enrichment in brain.
特异性：This assay has high sensitivity and excellent specificity for detection of Mouse TOLLIP. No significant cross-reactivity or interference between Mouse TOLLIP and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Mouse TOLLIP and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse TOLLIP in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse TOLLIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate TOLLIP in samples. An antibody specific for TOLLIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOLLIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOLLIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOLLIP bound in the initial step. The color development is stopped and the intensity of the color is measured.