Mouse Gastricsin (PGC) ELISA Kit

货号：	AE27842MO
样本：	Serum, Plasma, Other biological fluids
标记物：	Q9D7R7
适应物种：	Mouse (Mus musculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.063 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：PGC
别名：RP11-298J23.1; FLJ99563; PEPC; PGII; gastricsin|pepsin C|pepsinogen group II|preprogastricsin
检测范围：0.156-10 ng/mL
产品概述：Pepsinogen, the inactive precursor of pepsin belongs to the group of aspartic proteinases, is synthesized in the chief cells of gastric glands. Seven electrophoretically separable pepsinogens exist in human gastric mucosa. One group, called PGA or group I pepsinogen, is characterized by electrophoretically faster migration and localization in the fundus and body of the stomach; the second group, termed pepsinogen C, is localized in the whole stomach. PGCs are found in prostate and in seminal fluid whereas PGAs are not. the absence of detectable immunologic crossreactivity between the pepsinogens A and C results from divergent evolution of sequences located on the surface of the zymogens in contrast to the strongly conserved active site regions located within the binding cleft of the enzymes, inaccessible for antigenic recognition.
特异性：This assay has high sensitivity and excellent specificity for detection of Mouse PGC. No significant cross-reactivity or interference between Mouse PGC and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Mouse PGC and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse PGC in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse PGC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate PGC in samples. An antibody specific for PGC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGC bound in the initial step. The color development is stopped and the intensity of the color is measured.