Mouse Platelet Factor 4 (PF-4) ELISA Kit

货号：	AE27936MO
样本：	Serum, Plasma, Other biological fluids
标记物：	Q9Z126
适应物种：	Mouse (Mus musculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.068 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：PF-4
别名：CXCL4; MGC138298; SCYB4; chemokine (C-X-C motif) ligand 4|platelet factor 4 (chemokine (C-X-C motif) ligand 4)
检测范围：0.156-10 ng/mL
产品概述：Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family. This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation. It is usually found in a complex with proteoglycan.PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with a splice variant of the chemokine receptor CXCR3, known as CXCR3B. PF4 is the antigen in heparin-induced thrombocytopenia, an idiosyncratic autoimmune reaction to the administration of the anticoagulant heparin. PF4 autoantibodies have also been found in patients with thrombosis and features resembling HIT but no prior administration of heparin.
特异性：This assay has high sensitivity and excellent specificity for detection of Mouse PF-4. No significant cross-reactivity or interference between Mouse PF-4 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Mouse PF-4 and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse PF-4 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse PF-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate PF-4 in samples. An antibody specific for PF-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPF-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PF-4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PF-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.