Mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) ELISA Kit

货号：	AE26360MO
样本：	Serum, Plasma, Other biological fluids
标记物：	P62141
适应物种：	Mouse (Mus musculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	3.9 pg/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：PPP1CB
别名：MGC3672; PP-1B; PPP1CD; OTTHUMP00000200968|protein phosphatase 1; catalytic subunit; beta|protein phosphatase 1; catalytic subunit; delta isoform|protein phosphatase 1-beta|protein phosphatase 1-del
检测范围：15.6-1000 pg/mL
产品概述：Protein phosphatase-1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases involved in the dephosphorylation of a variety of proteins. These enzymes work in opposition to the protein kinases to control the level of phosphorylation. PP1 has 3 catalytic subunits, designated alpha, beta, and gamma. PP1-beta is also referred to as PP1-delta. PPP1Cb is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory.
特异性：This assay has high sensitivity and excellent specificity for detection of Mouse PPP1CB. No significant cross-reactivity or interference between Mouse PPP1CB and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Mouse PPP1CB and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse PPP1CB in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse PPP1CB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate PPP1CB in samples. An antibody specific for PPP1CB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP1CB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1CB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1CB bound in the initial step. The color development is stopped and the intensity of the color is measured.