Mouse Slit homolog 3 protein (SLIT3) ELISA Kit

货号：	AE18231MO
样本：	Serum, Plasma, Other biological fluids
标记物：	Q9WVB4
适应物种：	Mouse (Mus musculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.066 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：SLIT3
别名：FLJ10764; MEGF5; SLIL2; SLIT1; Slit-3; slit2; slit homolog 3
检测范围：0.156-10 ng/mL
产品概述：The SLIT3 cDNA encodes a 1,523-amino acid polypeptide with 41.1% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT3 gene was expressed as a major 5.5- and a minor 9.5-kb transcript primarily in the thyroid. SLIT1 and SLIT2 mRNAs were primarily expressed in the brain and spinal cord, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.
特异性：This assay has high sensitivity and excellent specificity for detection of Mouse SLIT3. No significant cross-reactivity or interference between Mouse SLIT3 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Mouse SLIT3 and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse SLIT3 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse SLIT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate SLIT3 in samples. An antibody specific for SLIT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLIT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.