Rat Coagulation factor IX (F9) ELISA Kit

货号：	AE61132RA
样本：	Serum, Plasma, Other biological fluids
标记物：	P16292
适应物种：	Rat (Rattus norvegicus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.067 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：F9
别名：RP6-88D7.1; FIX; HEMB; MGC129641; MGC129642; PTC; Christmas factor|factor 9|plasma thromboplastic component
检测范围：0.156-10 ng/mL
产品概述：Factor IX  is one of the serine proteases (EC 3.4.21.22) of the coagulation system; it belongs to peptidase family S2. Deficiency of this protein causes hemophilia B. It was discovered after a young boy named Stephen Christmas was found to be lacking this⃀Ϯ⁰Ϯ
特异性：This assay has high sensitivity and excellent specificity for detection of Rat F9. No significant cross-reactivity or interference between Rat F9 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Rat F9 and the recovery rates were calculated by comparing the measured value to the expected amount of Rat F9 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat F9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate F9 in samples. An antibody specific for F9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyF9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F9 bound in the initial step. The color development is stopped and the intensity of the color is measured.