Rat Klotho (KL) ELISA Kit

货号：	AE36648RA
样本：	Serum, Plasma, Other biological fluids
标记物：	Q9Z2Y9
适应物种：	Rat (Rattus norvegicus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.113 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：KL
别名：N/A
检测范围：0.312-20 ng/mL
产品概述：Klotho is a type-I membrane protein that is related to β-glucuronidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.Klotho is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. Its discovery was documented in 1997 by Kuro-o et al. The name of the gene comes from Klotho or Clotho, one of the Moirae, or Fates, in Greek mythology.
特异性：This assay has high sensitivity and excellent specificity for detection of Rat KL. No significant cross-reactivity or interference between Rat KL and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Rat KL and the recovery rates were calculated by comparing the measured value to the expected amount of Rat KL in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat KL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate KL in samples. An antibody specific for KL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KL bound in the initial step. The color development is stopped and the intensity of the color is measured.