Rat Oncostatin M (OSM) ELISA Kit

货号：	AE62891RA
样本：	Serum, Plasma, Other biological fluids
标记物：	Q65Z15
适应物种：	Rat (Rattus norvegicus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	6.5 pg/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：OSM
别名：MGC20461;
检测范围：15.6-1000 pg/mL
产品概述：Oncostatin M(OSM) is a cytokine originally isolated from medium conditioned by PMA-treated U-937 histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM.OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors.
特异性：This assay has high sensitivity and excellent specificity for detection of Rat OSM. No significant cross-reactivity or interference between Rat OSM and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Rat OSM and the recovery rates were calculated by comparing the measured value to the expected amount of Rat OSM in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat OSM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate OSM in samples. An antibody specific for OSM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyOSM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OSM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OSM bound in the initial step. The color development is stopped and the intensity of the color is measured.