Rat Thyroglobulin IgG antibody (TAb-IgG) ELISA Kit

货号：	AE62746RA
样本：	Serum, Plasma, Other biological fluids
适应物种：	Rat (Rattus norvegicus)
应用：	Qualitative
检测方法：	Competitive ELISA
检测限：	13.5 pg/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：TAb-IgG
别名：N/A
检测范围：31.25 -2000 pg/mL
产品概述：Thyroglobulin (Tg) is a 660 kDa, dimeric protein produced by and used entirely within the thyroid gland. In earlier literature, Tg was referred to as "colloid".Thyroglobulin should not be confused with Thyroxine-binding globulin, a carrier protein responsible for carrying the thyroid hormones in the blood.Tg is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3). The active form of triiodothyronine, 3, 5, 3' triiodothyronine, is produced both within the thyroid gland and periphery by 5'-deiodinase (which has been referred to as tetraiodothyronine 5' deiodinase.)Thyroglobulin levels in the blood can be used as a tumor marker for certain kinds of thyroid cancer. Thyroglobulin levels in the blood can also be elevated in cases of Graves' disease.
特异性：This assay has high sensitivity and excellent specificity for detection of Rat TAb-IgG. No significant cross-reactivity or interference between Rat TAb-IgG and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Rat TAb-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Rat TAb-IgG in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat TAb-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TAb-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated TAb-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled TAb-IgG and unlabeled TAb-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TAb-IgG in the sample. The color development is stopped and the intensity of the color is measured.