Rat Prolactin (PRL) ELISA Kit

货号：	AE25909RA
样本：	Serum, Plasma, Other biological fluids
标记物：	Q28632
适应物种：	Rat (Rattus norvegicus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.117 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：PRL
别名：LTH; Luteotropic Hormone
检测范围：0.312-20 ng/mL
产品概述：Luteotropic hormone is a peptide hormone discovered by Dr. Henry Friesen, primarily associated with lactation. In breastfeeding, the act of an infant suckling the nipple stimulates the production of prolactin, which fills the breast with milk via a process called lactogenesis, in preparation for the next feed. Oxytocin, another hormone, is also released, which triggers milk let-down.Prolactin (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of prolactin, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. Prolactin (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female.
特异性：This assay has high sensitivity and excellent specificity for detection of Rat PRL. No significant cross-reactivity or interference between Rat PRL and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Rat PRL and the recovery rates were calculated by comparing the measured value to the expected amount of Rat PRL in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat PRL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate PRL in samples. An antibody specific for PRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRL bound in the initial step. The color development is stopped and the intensity of the color is measured.