Rabbit Aggrecan core protein (ACAN) ELISA Kit

货号：	AE63217RB
样本：	Serum, Plasma, Other biological fluids
标记物：	Q28670
适应物种：	Rabbit (Oryctolagus cuniculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.239 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：ACAN
别名：AGC1; AGCAN; CSPG1; CSPGCP; MSK16; SEDK; cartilage-specific proteoglycan core protein|chondroitin sulfate proteoglycan 1|large aggregating proteoglycan
检测范围：0.625-40 ng/mL
产品概述：Aggrecan, or large aggregating proteoglycan, is a proteoglycan, or a protein modified with carbohydrates; the human form of the protein is 2316 amino acids long and can be expressed in multiple isoforms due to alternative splicing. Along with Type-II collagen, aggrecan forms a major structural component of cartilage, particularly articular cartilage.Aggrecan consists of two globular structural domains at the N-terminal end and one globular domain at the C-terminal end, separated by a large domain heavily modified with glycosaminoglycans. The two main modifier moieties are themselves arranged into distinct regions, a chondroitin sulfate and a keratan sulfate region.
特异性：This assay has high sensitivity and excellent specificity for detection of Rabbit ACAN. No significant cross-reactivity or interference between Rabbit ACAN and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Rabbit ACAN and the recovery rates were calculated by comparing the measured value to the expected amount of Rabbit ACAN in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rabbit ACAN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate ACAN in samples. An antibody specific for ACAN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAN bound in the initial step. The color development is stopped and the intensity of the color is measured.